Krauter K, Montgomery K, Yoon S J, LeBlanc-Straceski J, Renault B, Marondel I, Herdman V, Cupelli L, Banks A, Lieman J
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Nature. 1995 Sep 28;377(6547 Suppl):321-33. doi: 10.1038/377321a0.
Human chromosome 12 constitutes approximately 4.5% of the human genome and has an estimated size of 135 million base pairs (Mb). We have started to construct a high-resolution physical map of chromosome 12 as overlapping yeast artificial chromosomes (YACs), using as a foundation the first-generation physical map of this chromosome covers nearly 102 Mb of DNA and includes 426 highly polymorphic, monomorphic and gene-based markers. We also mapped 119 of the YACs, most of which are part of the physical map, by cytogenetic methods. Thus the map integrates genetic, physical and cytogenetic data and provides information about the organization of this chromosome and will help in the localization and cloning of disease-related genes. The strategy used here to generate the chromosome-12 map could be applied for the rapid construction of physical and expression maps for other human chromosomes.
人类12号染色体约占人类基因组的4.5%，估计大小为1.35亿个碱基对（Mb）。我们已开始构建12号染色体的高分辨率物理图谱，以重叠酵母人工染色体（YAC）为基础，该染色体的第一代物理图谱覆盖了近1.02亿碱基对的DNA，包括426个高度多态性、单态性和基于基因的标记。我们还通过细胞遗传学方法对119个YAC进行了定位，其中大多数是物理图谱的一部分。因此，该图谱整合了遗传、物理和细胞遗传学数据，提供了有关该染色体组织的信息，并将有助于疾病相关基因的定位和克隆。这里用于生成12号染色体图谱的策略可应用于快速构建其他人类染色体的物理图谱和表达图谱。
