一张覆盖小鼠基因组的第一代全基因组辐射杂种图谱。
A first-generation whole genome-radiation hybrid map spanning the mouse genome.
作者信息
McCarthy L C, Terrett J, Davis M E, Knights C J, Smith A L, Critcher R, Schmitt K, Hudson J, Spurr N K, Goodfellow P N
机构信息
Genetics Department, University of Cambridge, Downing Site, Cambridge CB2 3EH, UK.
出版信息
Genome Res. 1997 Dec;7(12):1153-61. doi: 10.1101/gr.7.12.1153.
Abstract
We have assembled a first-generation anchor map of the mouse genome using a panel of 94 whole-genome-radiation hybrids (WG-RHs) and 271 sequence-tagged sites (STSs). This is the first genome-wide RH anchor map of a model organism. All of the STSs have been previously localized on the genetic map and are located 8.8 Mb apart on average. This mouse WG-RH panel, known as T31, has an average retention frequency of 27.6% and an estimated potential resolution of 145 kb, making it a powerful resource for efficient large-scale expressed sequence tag mapping. [All of the mapping data for the maps presented here have been deposited at the Research Genetics, Inc., web site and can be freely accessed and downloaded at http://www.resgen.com/.]
摘要
我们利用一组94个全基因组辐射杂种细胞（WG-RHs）和271个序列标签位点（STSs）构建了小鼠基因组的第一代锚定图谱。这是首个模式生物的全基因组辐射杂种锚定图谱。所有的STSs先前已定位在遗传图谱上，平均间距为8.8 Mb。这个名为T31的小鼠WG-RH细胞系平均保留频率为27.6%，估计潜在分辨率为145 kb，使其成为高效大规模表达序列标签定位的强大资源。[此处展示的所有图谱的定位数据已存于Research Genetics, Inc.网站，可通过http://www.resgen.com/免费访问和下载。]
相似文献
1
A first-generation whole genome-radiation hybrid map spanning the mouse genome.一张覆盖小鼠基因组的第一代全基因组辐射杂种图谱。
Genome Res. 1997 Dec;7(12):1153-61. doi: 10.1101/gr.7.12.1153.
2
A radiation hybrid map of 95 STSs spanning human chromosome 13q.跨越人类13号染色体长臂的95个序列标签位点（STSs）的辐射杂种图谱。
Genomics. 1995 Jun 10;27(3):502-10. doi: 10.1006/geno.1995.1083.
3
An STS-based radiation hybrid map of the human genome.基于序列标签位点的人类基因组辐射杂种图谱。
Genome Res. 1997 May;7(5):422-33. doi: 10.1101/gr.7.5.422.
4
Regional assignment of human ESTs by whole-genome radiation hybrid mapping.通过全基因组辐射杂种图谱对人类EST进行区域定位。
Mamm Genome. 1996 Jun;7(6):446-50. doi: 10.1007/s003359900130.
5
A radiation hybrid map of human chromosome 18.人类18号染色体的辐射杂种图谱。
Cytogenet Cell Genet. 1994;66(3):196-213. doi: 10.1159/000133697.
6
A radiation hybrid map of human chromosome 5 with integration of cytogenetic, genetic, and transcript maps.整合了细胞遗传学、遗传学和转录图谱的人类5号染色体辐射杂种图谱。
Genome Res. 1997 Sep;7(9):897-909. doi: 10.1101/gr.7.9.897.
7
An integrated transcript map of human chromosome 1p35-p36.人类染色体1p35 - p36的综合转录图谱
Genomics. 1997 May 15;42(1):126-36. doi: 10.1006/geno.1997.4714.
8
Cross-referencing radiation hybrid data to the recombination map: lessons from mouse chromosome 18.将辐射杂种数据与重组图谱进行交叉参照：来自小鼠18号染色体的经验教训。
Genomics. 2000 Oct 1;69(1):27-36. doi: 10.1006/geno.2000.6314.
9
A high-resolution whole genome radiation hybrid map of human chromosome 17q22-q25.3 across the genes for GH and TK.跨越生长激素（GH）和胸苷激酶（TK）基因的人类17号染色体q22 - q25.3区域的高分辨率全基因组辐射杂种图谱。
Genomics. 1996 Apr 15;33(2):185-92. doi: 10.1006/geno.1996.0182.
10
High-density radiation hybrid map of human chromosome 18 and contig of 18p.人类18号染色体的高密度辐射杂种图谱及18p的重叠群
Genomics. 1996 Oct 1;37(1):9-18. doi: 10.1006/geno.1996.0514.
引用本文的文献
1
Construction of two whole genome radiation hybrid panels for dromedary (Camelus dromedarius): 5000 and 15000.构建两个全基因组辐射杂种面板用于单峰驼（Camelus dromedarius）：5000 和 15000。
Sci Rep. 2018 Jan 31;8(1):1982. doi: 10.1038/s41598-018-20223-5.
2
Mapping and genotypic analysis of the NK-lysin gene in chicken.鸡自然杀伤细胞溶素基因的定位与基因型分析
Genet Sel Evol. 2014 Jul 7;46(1):43. doi: 10.1186/1297-9686-46-43.
3
Endosperm tolerance of paternal aneuploidy allows radiation hybrid mapping of the wheat D-genome and a measure of γ ray-induced chromosome breaks.胚乳对父源非整倍体的耐受性使得可以对小麦 D 基因组进行辐射杂种作图，并测量γ射线诱导的染色体断裂。
PLoS One. 2012;7(11):e48815. doi: 10.1371/journal.pone.0048815. Epub 2012 Nov 7.
4
Construction of whole genome radiation hybrid panels and map of chromosome 5A of wheat using asymmetric somatic hybridization.利用不对称体细胞杂交构建全基因组辐射杂种面板和小麦 5A 染色体图谱。
PLoS One. 2012;7(7):e40214. doi: 10.1371/journal.pone.0040214. Epub 2012 Jul 16.
5
A genome-wide map of human genetic interactions inferred from radiation hybrid genotypes.从辐射杂种基因型推断的人类遗传相互作用的全基因组图谱。
Genome Res. 2010 Aug;20(8):1122-32. doi: 10.1101/gr.104216.109. Epub 2010 May 27.
6
Directed mammalian gene regulatory networks using expression and comparative genomic hybridization microarray data from radiation hybrids.利用辐射杂种的表达和比较基因组杂交微阵列数据构建的定向哺乳动物基因调控网络。
PLoS Comput Biol. 2009 Jun;5(6):e1000407. doi: 10.1371/journal.pcbi.1000407. Epub 2009 Jun 12.
7
Targeted disruption of the mouse Csrp2 gene encoding the cysteine- and glycine-rich LIM domain protein CRP2 result in subtle alteration of cardiac ultrastructure.对编码富含半胱氨酸和甘氨酸的LIM结构域蛋白CRP2的小鼠Csrp2基因进行靶向破坏，会导致心脏超微结构发生细微改变。
BMC Dev Biol. 2008 Aug 19;8:80. doi: 10.1186/1471-213X-8-80.
8
Fine mapping of regulatory loci for mammalian gene expression using radiation hybrids.利用辐射杂种细胞对哺乳动物基因表达调控位点进行精细定位。
Nat Genet. 2008 Apr;40(4):421-9. doi: 10.1038/ng.113. Epub 2008 Mar 23.
9
Radiation hybrid maps of Medaka chromosomes LG 12, 17, and 22.青鳉鱼第12、17和22号染色体的辐射杂种图谱。
DNA Res. 2007 Jun 30;14(3):135-40. doi: 10.1093/dnares/dsm012. Epub 2007 Jun 25.
10
High-resolution radiation hybrid map of wheat chromosome 1D.小麦1D染色体的高分辨率辐射杂种图谱。
Genetics. 2006 Jun;173(2):1089-99. doi: 10.1534/genetics.106.056481. Epub 2006 Apr 19.
本文引用的文献
1
Whole genome radiation hybrid mapping.全基因组辐射杂种图谱绘制
Trends Genet. 1996 Dec;12(12):491-3. doi: 10.1016/s0168-9525(96)30110-8.
2
Functional identification of the mouse circadian Clock gene by transgenic BAC rescue.通过转基因细菌人工染色体（BAC）拯救对小鼠生物钟基因进行功能鉴定。
Cell. 1997 May 16;89(4):655-67. doi: 10.1016/s0092-8674(00)80246-9.
3
An STS-based radiation hybrid map of the human genome.基于序列标签位点的人类基因组辐射杂种图谱。
Genome Res. 1997 May;7(5):422-33. doi: 10.1101/gr.7.5.422.
4
Isolation and characterization of a mouse homolog of the X-linked ocular albinism (OA1) gene.X连锁眼白化病（OA1）基因小鼠同源物的分离与鉴定。
Genomics. 1996 Oct 15;37(2):219-25. doi: 10.1006/geno.1996.0545.
5
Genomic organization of the mottled gene, the mouse homologue of the human Menkes disease gene.斑驳基因的基因组结构，人类门克斯病基因的小鼠同源物。
Genomics. 1996 Oct 1;37(1):96-104. doi: 10.1006/geno.1996.0525.
6
A radiation hybrid map of the human genome.人类基因组的辐射杂种图谱。
Hum Mol Genet. 1996 Mar;5(3):339-46. doi: 10.1093/hmg/5.3.339.
7
A gene map of the human genome.人类基因组的基因图谱。
Science. 1996 Oct 25;274(5287):540-6.
8
Isolation and chromosomal mapping of a mouse homolog of the Batten disease gene CLN3.巴顿病基因CLN3的小鼠同源基因的分离与染色体定位。
Genomics. 1996 Aug 1;35(3):617-9. doi: 10.1006/geno.1996.0410.
9
The mammalian single-minded (SIM) gene: mouse cDNA structure and diencephalic expression indicate a candidate gene for Down syndrome.哺乳动物单 minded（SIM）基因：小鼠cDNA结构和间脑表达表明它是唐氏综合征的一个候选基因。
Genomics. 1996 Jul 1;35(1):136-43. doi: 10.1006/geno.1996.0332.
10
Construction of a mouse whole-genome radiation hybrid panel and application to MMU11.小鼠全基因组辐射杂种板的构建及其在MMU11上的应用。
Genomics. 1996 Jun 1;34(2):193-7. doi: 10.1006/geno.1996.0265.
Zotero 插件