Shuttlesworth KL, Cerniglia CE
Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, 3900 NCTR Rd., Jefferson, AR 72079, USA
Microb Ecol. 1996 May;31(3):305-17.
Phenanthrene-degrading bacteria were isolated from enrichment cultures of soils contaminated with creosote and jet fuel. The isolates from the creosote enrichments were classified by fatty acid methyl ester profiles as Acidovorax delafieldii and Sphingomonas paucimobilis; the bacterium from the jet fuel-contaminated soil was not identified and was designated strain JFD11. All three isolates used phenanthrene as a sole carbon and energy source, and two of the isolates used fluoranthene as a sole carbon and energy source. Anthracene and fluorene were cometabolized by all three strains, but pyrene was not transformed. Naphthalene inhibited all of the strains, and 28-h cultures of A. delafieldii were inhibited by naphthalene concentrations as low as 5 ppm. Short-term degradation experiments were undertaken with center-well flasks and concentrations of phenanthrene ranging from 1.2 to 12.0 &mgr;M. Since initial degradation rates were not a function of phenanthrene concentration, it was inferred that the half-saturation constants were less than the lowest phenanthrene concentration tested.
从受杂酚油和喷气燃料污染的土壤富集培养物中分离出菲降解细菌。根据脂肪酸甲酯谱，杂酚油富集培养物中的分离菌株被归类为德氏食酸菌（Acidovorax delafieldii）和少动鞘氨醇单胞菌（Sphingomonas paucimobilis）；来自喷气燃料污染土壤的细菌未被鉴定，被命名为菌株JFD11。所有三株分离菌都以菲作为唯一的碳源和能源，其中两株分离菌还以荧蒽作为唯一的碳源和能源。蒽和芴被所有三株菌株共代谢，但芘未被转化。萘抑制所有菌株，德氏食酸菌28小时的培养物在萘浓度低至5 ppm时就受到抑制。使用中心孔板进行了短期降解实验，菲的浓度范围为1.2至12.0 μM。由于初始降解速率不是菲浓度的函数，因此推断半饱和常数小于测试的最低菲浓度。
