Chowdhary B P, Frönicke L, Gustavsson I, Scherthan H
Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 7023, S-750 07 Uppsala, Sweden.
Mamm Genome. 1996 Apr;7(4):297-302. doi: 10.1007/s003359900086.
Comparative chromosome painting with individual human chromosome-specific libraries (CSLs) on cattle metaphase chromosomes delineated 46 homologous chromosomal segments between the two species. Continuous arrangement of these segments on individual cattle chromosomes demonstrates a nearly complete coverage of the bovine karyotype and shows physical boundaries of bovine chromosomal segments homologous to individual human chromosomes. Alignment of the available comparative gene mapping data with the homologous segments strongly supports the detected gross homologies between the karyotypes of the two species. In addition to cattle, four human CSLs were hybridized to sheep metaphase chromosomes also, to further verify the known karyotype homology within the Bovidae. Besides its application to karyotype evolution research, the comparative knowledge provides for rapid expansion of the much needed Type I locus-based bovine gene map.
利用人类个体染色体特异性文库（CSLs）对牛中期染色体进行比较染色体描绘，确定了两个物种之间的46个同源染色体片段。这些片段在单个牛染色体上的连续排列表明几乎完全覆盖了牛的核型，并显示了与人类单个染色体同源的牛染色体片段的物理边界。将现有的比较基因图谱数据与同源片段进行比对，有力地支持了所检测到的两个物种核型之间的总体同源性。除了牛之外，还将四个人类CSLs与绵羊中期染色体进行杂交，以进一步验证牛科动物中已知的核型同源性。除了应用于核型进化研究外，这种比较知识还为急需的基于I型基因座的牛基因图谱的快速扩展提供了条件。
