Owen-Hughes T, Utley R T, Côté J, Peterson C L, Workman J L
Department of Biochemistry and Molecular Biology and Center for Gene Regulation, Pennsylvania State University, University Park, PA 16802-4500, USA.
Science. 1996 Jul 26;273(5274):513-6. doi: 10.1126/science.273.5274.513.
The SWI/SNF complex participates in the restructuring of chromatin for transcription. The function of the yeast SWI/SNF complex in the remodeling of a nucleosome array has now been analyzed in vitro. Binding of the purified SWI/SNF complex to a nucleosome array disrupted multiple nucleosomes in an adenosine triphosphate-dependent reaction. However, removal of SWI/SNF left a deoxyribonuclease I-hypersensitive site specifically at a nucleosome that was bound by derivatives of the transcription factor Gal4p. Analysis of individual nucleosomes revealed that the SWI/SNF complex catalyzed eviction of histones from the Gal4-bound nucleosomes. Thus, the transient action of the SWI/SNF complex facilitated irreversible disruption of transcription factor-bound nucleosomes.
SWI/SNF复合物参与染色质重构以促进转录。目前已在体外分析了酵母SWI/SNF复合物在核小体阵列重塑中的功能。纯化的SWI/SNF复合物与核小体阵列的结合在依赖三磷酸腺苷的反应中破坏了多个核小体。然而，去除SWI/SNF后，在一个由转录因子Gal4p衍生物结合的核小体处特异性地留下了一个脱氧核糖核酸酶I超敏位点。对单个核小体的分析表明，SWI/SNF复合物催化组蛋白从Gal4结合的核小体上被逐出。因此，SWI/SNF复合物的短暂作用促进了转录因子结合的核小体的不可逆破坏。
