Weinberg D H, Sirinathsinghji D J, Tan C P, Shiao L L, Morin N, Rigby M R, Heavens R H, Rapoport D R, Bayne M L, Cascieri M A, Strader C D, Linemeyer D L, MacNeil D J
Department of Molecular Pharmacology and Biochemistry, Merck Research Laboratories, Rahway, New Jersey 07065-0900, USA.
J Biol Chem. 1996 Jul 12;271(28):16435-8. doi: 10.1074/jbc.271.28.16435.
The neuropeptide Y family of peptides, which includes neuropeptide Y (NPY), peptide YY (PYY), and pancreatic polypeptide (PP), are found in the central and peripheral nervous system and display a wide array of biological activities. These actions are believed to be mediated through pharmacologically distinct G protein-coupled receptors, and, to date, three members of the NPY receptor family have been cloned. In this study we describe the cloning and expression of a novel NPY receptor from mouse genomic DNA. This receptor, designated NPY Y5, shares 60% amino acid identity to the murine NPY Y1 receptor. The pharmacology of this novel receptor resembles that of the NPY Y1 receptor and is distinct from that described for the NPY Y2, Y3, and Y4 receptors. In situ hybridization of mouse brain sections reveals expression of this receptor within discrete regions of the hypothalamus including the suprachiasmatic nucleus, anterior hypothalamus, bed nucleus stria terminalis, and the ventromedial nucleus with no localization apparent elsewhere in the brain.
神经肽 Y 家族的肽类，包括神经肽 Y（NPY）、肽 YY（PYY）和胰多肽（PP），存在于中枢和外周神经系统中，并表现出广泛的生物活性。这些作用被认为是通过药理学上不同的 G 蛋白偶联受体介导的，迄今为止，NPY 受体家族的三个成员已被克隆。在本研究中，我们描述了从小鼠基因组 DNA 中克隆和表达一种新型 NPY 受体。这种受体被命名为 NPY Y5，与小鼠 NPY Y1 受体有 60%的氨基酸同一性。这种新型受体的药理学特性类似于 NPY Y1 受体，与 NPY Y2、Y3 和 Y4 受体所描述的不同。小鼠脑切片的原位杂交显示该受体在下丘脑的离散区域表达，包括视交叉上核、下丘脑前部、终纹床核和腹内侧核，在大脑其他部位未发现明显定位。
