Kato K, Kato J, Hodgson W J, Abraham N G, Onodera K, Imai M, Kasai S, Mito M
Department of Medicine and Gastrointestinal Surgery, New York Medical College, Valhalla 10595, USA.
Cell Transplant. 1997 Sep-Oct;6(5):531-4. doi: 10.1177/096368979700600516.
We examined the expression and enzymatic activity of cytochrome P450 LA omega within transplanted hepatocytes. Fetal hepatocytes were harvested at day 20 of gestation from spontaneously hypertensive rats (SHRs) and transplanted into recipient adult SHR spleens. Microscopic examination of the recipient spleens at 4 and 10 wk after transplantation revealed masses of hepatocytes with cord-like structures in the red pulp. Immunochemical studies detected cytochrome (cyto) P450 LA omega in the fetal hepatocytes before transplantation without prior induction. Although the cyto P450 LA omega was not detected by the second week after transplantation, by the 6th and 10th wk after transplantation, it was. Cyto P450-arachidonic acid omega/omega-1 hydroxylase activity (formation of 20- and 19-hydroxyeicosatetraenoic acid) was detected at 10 wk after transplantation, but not 2 or 6 wk after transplantation. These results demonstrated that fetal hepatocytes can be transplanted successfully into recipient spleens and then grow in the spleens, as in the case of the adult hepatocyte response.
我们检测了移植肝细胞中细胞色素P450 LA ω的表达及酶活性。在妊娠第20天从自发性高血压大鼠（SHR）获取胎肝细胞，并将其移植到成年受体SHR的脾脏中。移植后4周和10周对受体脾脏进行显微镜检查，发现红髓中有大量具有索状结构的肝细胞。免疫化学研究在未预先诱导的情况下，于移植前在胎肝细胞中检测到细胞色素（cyto）P450 LA ω。虽然移植后第二周未检测到细胞色素P450 LA ω，但在移植后第6周和第10周检测到了。在移植后10周检测到细胞色素P450 - 花生四烯酸ω/ω - 1羟化酶活性（20 - 和19 - 羟基二十碳四烯酸的形成），但在移植后2周和6周未检测到。这些结果表明，胎肝细胞能够成功移植到受体脾脏中，并随后在脾脏中生长，如同成年肝细胞的反应情况一样。
