Kato K, Onodera K, Sakata H, Imai M, Kato J, Hodgson W J, Abraham N G, Kasai S, Mito M
Department of Gastrointestinal Surgery, New York Medical College, Valhalla 10595, USA.
Transplantation. 1998 Aug 27;66(4):441-5. doi: 10.1097/00007890-199808270-00005.
The expression and enzymatic activity of the cytochrome P450 LAomega within intrasplenically transplanted hepatocytes was investigated.
Fetal hepatocytes were harvested from spontaneously hypertensive rats and transplanted into recipient adult spontaneously hypertensive rat spleens.
Microscopic examination revealed masses of hepatocytes in the red pulp. Immunochemical studies detected cytochrome P450 LAomega in transplanted hepatocytes by 6 and 10 weeks after transplantation. Cytochrome P450 LAomega mRNA accumulates at 6 weeks after transplantation. Cytochrome P450-arachidonic acid omega/omega-1 hydroxylase activity (formation of 20/19-hydroxyeicosatetraenoic acid) was detected at 10 weeks after transplantation.
These results demonstrated that fetal hepatocytes grow in the spleen and function similarly to adult hepatocytes.
研究了脾内移植的肝细胞中细胞色素P450 LAomega的表达和酶活性。
从自发性高血压大鼠中获取胎儿肝细胞，并将其移植到成年受体自发性高血压大鼠的脾脏中。
显微镜检查显示红髓中有大量肝细胞。免疫化学研究在移植后6周和10周检测到移植肝细胞中的细胞色素P450 LAomega。细胞色素P450 LAomega mRNA在移植后6周积累。在移植后10周检测到细胞色素P450 - 花生四烯酸ω/ω-1羟化酶活性（20/19-羟基二十碳四烯酸的形成）。
这些结果表明胎儿肝细胞在脾脏中生长，并且功能与成年肝细胞相似。
