Goso Y, Ogata Y, Ishihara K, Hotta K
Department of Biochemistry, Kitasato University School of Medicine, Sagamihara, Japan.
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1996 Jan;113(1):17-21. doi: 10.1016/0742-8413(95)02042-x.
The effect of the traditional herbal medicine, Rikkunshi-to and its component crude drugs, Zingiberis Rhizoma and Glycyrrhizae Radix, on the gastric mucin was studied using a method developed to separate and quantify the mucin localized in the different layers of rat gastric mucosa. The oral administration of spray-dried extract to Rikkunshi-to (1000 mg/kg), Zingiberis Rhizoma (500 mg/kg) and Glycyrrhizae Radix (500 mg/kg) significantly prevented gastric mucosal damage induced by 70% ethanol in rats. In ethanol-treated rats the mucin content of the deep mucosa was reduced, and the reduction of the deep corpus mucin content was significantly inhibited by pretreatment of Rikkunshi-to and Zingiberis Rhizoma. Rikkunshi-to and Glycyrrhizae Radix pretreatment increased the surface mucin content by 140 and 146%, respectively. The effect on the gastric mucin by each drug differed in the different layers of the gastric mucosa.
采用一种专门开发的方法，用于分离和定量大鼠胃黏膜不同层中定位的黏蛋白，研究了传统草药六君子汤及其组成生药干姜和甘草对胃黏蛋白的影响。给大鼠口服六君子汤喷雾干燥提取物（1000mg/kg）、干姜（500mg/kg）和甘草（500mg/kg），可显著预防70%乙醇诱导的大鼠胃黏膜损伤。在乙醇处理的大鼠中，深层黏膜的黏蛋白含量降低，六君子汤和干姜预处理可显著抑制深层胃体黏蛋白含量的降低。六君子汤和甘草预处理分别使表面黏蛋白含量增加了140%和146%。每种药物对胃黏蛋白的影响在胃黏膜的不同层中有所不同。
