Chinery R, Poulsom R, Cox H M
Histopathology Unit, Imperial Cancer Research Fund, London, UK.
Gene. 1996 Jun 1;171(2):249-53. doi: 10.1016/0378-1119(96)00074-1.
Trefoil peptides, a growing family of secretory molecules, have been identified mainly in the gastrointestinal tract of humans, rodents and amphibians. In the present study, the nucleotide sequence of a large portion (81%) of the gene encoding murine intestinal trefoil factor (mITF) and its whole genomic organization were determined. The mITF gene contains three exons distributed over 5 kb of genomic DNA. The genomic sequence is highly conserved, as compared with that of the rat and human ITF, and contains several AP-1-binding sites, the consensus binding site for the transcription factor Sp1, and a sequence homologous to a heat-shock element. Fluorescence in situ hybridization was used to assign ITF to chromosome 17 of the murine genome, a region syntenic with the trefoil gene cluster on human chromosome 21q22.3.
三叶肽是一类不断增加的分泌分子家族，主要在人类、啮齿动物和两栖动物的胃肠道中被发现。在本研究中，确定了编码小鼠肠三叶因子（mITF）的基因的一大部分（81%）的核苷酸序列及其整个基因组结构。mITF基因包含三个外显子，分布在5 kb的基因组DNA上。与大鼠和人类ITF的基因组序列相比，该基因组序列高度保守，包含几个AP-1结合位点、转录因子Sp1的共有结合位点以及一个与热休克元件同源的序列。荧光原位杂交被用于将ITF定位到小鼠基因组的17号染色体上，该区域与人类21号染色体q22.3上的三叶基因簇同线。
