van Raamsdonk M, de Soet J J, de Graaff J
Academic Centre for Dentistry, Amsterdam, The Netherlands.
Caries Res. 1993;27(1):31-7. doi: 10.1159/000261512.
Local passive immunization has been suggested as a method of preventing colonization of teeth by mutans streptococci. In this study we describe the effect of local application of monoclonal antibodies (Mabs) on the colonization of Streptococcus sobrinus. The rats, 37 days old, were divided in 4 groups: group 1 received Mab OMVU10 (Mab reactive with Antigen B of S. sobrinus, IgG2b), group 2 received Clone 24 (Mab reactive with lipid A of Escherichia coli, IgG2b), group 3 received uninoculated culture medium, and group 4 received phosphate-buffered saline (PBS). Mabs were applied 6 times on the surfaces of the molars and the labial surfaces of the incisors of the rats, on days 37, 40, 44, 46, 49 and 54 after birth. After the third application of Mabs, all rats were inoculated with S. sobrinus (day 45). Subsequently, the rats were fed a cariogenic diet containing 20% sucrose and 5% glucose. The rats were killed 34 days after inoculation and the maxillary molars were extracted and homogenized in order to determine the numbers of S. sobrinus. The proportions of S. sobrinus, expressed as a percentage of the total cultivable microflora, in rats which received OMVU10 (group 1) were 23.1 +/- 14.8%, whereas in rats which received Clone 24, culture medium or PBS the levels were 34.5 +/- 11.3%, 40.7 +/- 14.9% and 36.7 +/- 9.7%, respectively. Statistical analysis showed that the level of colonization of rats which received OMVU10 was significantly lower than that of rats which received Clone 24, culture medium or PBS (p < 0.001, p < 0.013 and p < 0.01, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)
局部被动免疫已被提议作为一种预防变形链球菌在牙齿上定植的方法。在本研究中，我们描述了局部应用单克隆抗体（Mab）对远缘链球菌定植的影响。将37日龄的大鼠分为4组：第1组接受Mab OMVU10（与远缘链球菌抗原B反应的Mab，IgG2b），第2组接受克隆24（与大肠杆菌脂多糖反应的Mab，IgG2b），第3组接受未接种的培养基，第4组接受磷酸盐缓冲盐水（PBS）。在出生后的第37、40、44、46、49和54天，将Mab应用于大鼠磨牙表面和切牙唇面6次。在第三次应用Mab后，所有大鼠均接种远缘链球菌（第45天）。随后，给大鼠喂食含20%蔗糖和5%葡萄糖的致龋饮食。接种后34天处死大鼠，提取上颌磨牙并匀浆以确定远缘链球菌的数量。接受OMVU10的大鼠（第1组）中远缘链球菌的比例，以可培养微生物总数的百分比表示为23.1±14.8%，而接受克隆24、培养基或PBS的大鼠中该水平分别为34.5±11.3%、40.7±14.9%和36.7±9.7%。统计分析表明，接受OMVU10的大鼠的定植水平显著低于接受克隆24、培养基或PBS的大鼠（分别为p<0.001、p<（此处原文有误，推测应为p<0.013）和p<0.01）。（摘要截断于250字）
