Crespo J, Dueñas C, Sánchez-Antolín G, Fábrega E, Carte B, Pons-Romero F
Servicio de Aparato Digestivo, Hospital Universitario Marqués de Valdecilla, Santander.
Med Clin (Barc). 1996 Feb 24;106(7):241-5.
The prevalence of HBV and HCV infections in patients with hepatocellular carcinoma may be related to variations in the geographic area of study. For this reason, we have analized the relative prevalence of HBV and HCV infections in 94 patients with hepatocellular carcinoma from Cantabria (North of Spain).
We have studied 94 patients with hepatocellular carcinoma from January 1988 to December 1993. Commercially available radioimmunoassay or ELISA were used for detection of HBsAg, anti-HBs and anti-HBc. The HBV DNA was analized by PCR. The HCV infection was assayed by ELISA-2 and RT-PCR.
The HBV infection was detected in 27 patients: 19 patients were HBsAg positive and 8 patients HBsAg negative, anti-HBc positive, DNA HBV positive by PCR. The HCV infection was found in 57 patients. Forty patients were infected with both viruses. Of the remain twenty-four, forty were alcoholics. We found in 61 patients more than one etiological factor. Hepatoma was the first manifestation of liver disease in 24 cases and these were more frequently in HCV than in those with HBV infection. Moreover, the first group were older and have lower alcohol intake.
肝细胞癌患者中乙肝病毒（HBV）和丙肝病毒（HCV）感染的患病率可能与研究的地理区域差异有关。因此，我们分析了来自西班牙北部坎塔布里亚的94例肝细胞癌患者中HBV和HCV感染的相对患病率。
我们研究了1988年1月至1993年12月期间的94例肝细胞癌患者。使用市售的放射免疫分析或酶联免疫吸附测定（ELISA）检测乙肝表面抗原（HBsAg）、乙肝表面抗体（抗-HBs）和乙肝核心抗体（抗-HBc）。通过聚合酶链反应（PCR）分析HBV DNA。通过ELISA-2和逆转录PCR（RT-PCR）检测HCV感染。
在27例患者中检测到HBV感染：19例患者HBsAg阳性，8例患者HBsAg阴性、抗-HBc阳性且PCR检测HBV DNA阳性。在57例患者中发现HCV感染。40例患者同时感染了两种病毒。其余24例中，40例为酗酒者。我们在61例患者中发现了不止一种病因。肝癌是24例肝病的首发表现，这些患者中HCV感染比HBV感染更为常见。此外，第一组患者年龄较大且酒精摄入量较低。
1）在西班牙坎塔布里亚，大多数肝细胞癌病例与HBV、HCV和酒精有关。2）通过PCR分析HBV DNA和HCV RNA可诊断两种病毒的隐匿感染，尤其是在HBV和HCV合并感染的病例中。3）在高比例的病例中，肝癌是肝病的首发表现。
