Sakharov R S, Nofal' Kh K, Druz' A F, Shchupik Iu P
Sud Med Ekspert. 1996 Jan-Mar;39(1):23-7.
Group a and b isohemagglutinins are much better detected in blood stains by enzyme-treated, but not native red cells. This is specially true for antibodies of poorly dissolved strains, whose group antibodies can be detected virtually only by enzyme-treated red cells in papain extracts possessing the highest immunological activity. Two sensitive methods for the detection of isohemagglutinins have been developed: extract titration and modified Lattes' method, permitting a reliable detection of group antibodies in old blood stains, undetectable by routine methods.
通过酶处理而非天然红细胞，能更好地在血痕中检测到A组和B组同种血凝素。对于溶解性差的菌株的抗体而言尤其如此，其血型抗体实际上只能通过具有最高免疫活性的木瓜蛋白酶提取物中的酶处理红细胞来检测。现已开发出两种检测同种血凝素的灵敏方法：提取物滴定法和改良的拉泰斯氏法，这两种方法能够可靠地检测出陈旧血痕中的血型抗体，而常规方法无法检测到这些抗体。
