Sakharov R S, Fedulova M V, Gureeva N B, Nofal' Kh K, Druz' A F, Shupik Iu P
Sud Med Ekspert. 1998 Mar-Apr;41(2):40-4.
The sensitivity of the absorption-elution test (AET) for the detection of A, B, and H antigens in blood traces can be appreciably increased by using serologically active proteases at different stages of the test. A sensitive AET modification is developed, detecting group antigens in far lesser amounts of the material than by routine AET without proteases. A higher sensitivity of the antigen-antibody reaction between enzyme-treated erythrocytes and tested blood traces permits the use of standard group-specific sera less active than usually; the actually solves the problem of deficit of the sera fit for this test.
在吸收-洗脱试验（AET）检测血痕中A、B和H抗原的过程中，通过在试验的不同阶段使用具有血清学活性的蛋白酶，可显著提高该试验的灵敏度。开发了一种灵敏的AET改良方法，与未使用蛋白酶的常规AET相比，该方法能在少得多的材料中检测到血型抗原。酶处理红细胞与待测血痕之间抗原-抗体反应的更高灵敏度允许使用活性低于通常水平的标准血型特异性血清；这实际上解决了适用于该试验的血清短缺问题。
