[Detecting ABO blood type of bloodstain with fluorescent antibody method].

UNLABELLED

OBJECTIVE To explore the advantage and feasibility of fluorescent antibody method for detection of blood type in biological material.

METHODS

According to theory of specific binding of antigen and antibody, at first the anti-A monoclonal antibody (MA) and anti-B MA were labeled with the fluorescent, then fluorescent-labeled antibodies (FLA) were bound with corresponding biological material (such as bloodstain) in the optimum condition, finally the ABO blood type of bloodstain was determined under microscope fluorescent.

RESULTS

The fluorescent antibody method is highly sensitive, accurate and simple.

CONCLUSION

The fluorescent antibody method is an accurate and reliable method for detection of ABO blood type in biological material.