Evans B S, Dudley C A, Klasson K T
Chemical Technology Division, Oak Ridge National Laboratory, TN 37831-6044, USA.
Appl Biochem Biotechnol. 1996 Spring;57-58:885-94. doi: 10.1007/BF02941769.
Many industrial locations have identified the need for treatment of polychlorinated biphenyl (PCB) wastes and remediation of PCB-contaminated sites. Biodegradation of PCBs is a potentially effective technology for treatment of PCB-contaminated soils and sludges; however, a practicable remediation technology has not yet been demonstrated. In laboratory experiments, soil slurry microcosms inoculated with microorganisms extracted from PCB-contaminated Hudson River sediments have been used for anaerobic dechlorination of weathered Aroclor 1248 in contaminated soil with a low organic carbon content. Anaerobic incubation was then followed by exposure to air, addition of biphenyl, and inoculation with Pseudomonas sp. LB400, an aerobic PCB degrader. The sequential anaerobic-aerobic treatment constituted an improvement compared to anaerobic or aerobic treatment alone by reducing the total amount of PCBs remaining and decreasing the tendency for end products to accumulate in humans. A 70% reduction of PCBs was observed during sequential treatment with products containing fewer chlorines and having a shorter half-life in humans than the original PCBs. The aerobic treatment alone was also quite effective as a stand-alone treatment reducing the PCBs by 67%. The results represent a case in which anaerobic river sediment organisms have been successfully transferred to a matrix free of river or lake sediments.
许多工业场所已认识到处理多氯联苯（PCB）废物以及修复受PCB污染场地的必要性。多氯联苯的生物降解是处理受PCB污染土壤和污泥的一种潜在有效技术；然而，尚未证明有切实可行的修复技术。在实验室实验中，接种从受PCB污染的哈德逊河沉积物中提取的微生物的土壤泥浆微观世界已被用于对低有机碳含量的受污染土壤中风化的Aroclor 1248进行厌氧脱氯。然后进行厌氧培养，之后暴露于空气中，添加联苯，并接种好氧PCB降解菌假单胞菌LB400。与单独的厌氧或好氧处理相比，顺序厌氧-好氧处理通过减少残留的多氯联苯总量以及降低最终产物在人体内积累的趋势而有所改进。在顺序处理过程中，观察到多氯联苯减少了70%，生成的产物含氯量更少，在人体内的半衰期比原始多氯联苯更短。单独的好氧处理作为一种独立处理方法也相当有效，可将多氯联苯减少67%。这些结果表明，厌氧河流沉积物中的微生物已成功转移到不含河流或湖泊沉积物的基质中。
