胰岛素和胰岛素样生长因子-1与粘着斑激酶同时刺激桩蛋白的去磷酸化。
Insulin and insulin-like growth factor-1 stimulate dephosphorylation of paxillin in parallel with focal adhesion kinase.
作者信息
Konstantopoulos N, Clark S
机构信息
University of Melbourne, Department of Medicine, Parkville, Victoria, Australia.
出版信息
Biochem J. 1996 Mar 1;314 ( Pt 2)(Pt 2):387-90. doi: 10.1042/bj3140387.
Abstract
Paxillin and focal adhesion kinase (pp125FAK) co-localize in focal adhesions; recently insulin has been shown to stimulate the dephosphorylation of pp125FAK; however, its effect on paxillin is unknown. We show that insulin and IGF-1 can stimulate the dephosphorylation of paxillin in CHOT (overexpress human insulin receptors) and CHO delta CT69 (overexpress insulin receptors lacking C-terminal 69 amino acids) cells. Furthermore, the insulin-receptor C-terminus is not needed for either insulin or IGF-1 to stimulate paxillin or pp125FAK dephosphorylation in the CHO (Chinese-hamster ovary) cell lines used.
摘要
桩蛋白和粘着斑激酶（pp125FAK）共定位于粘着斑；最近有研究表明胰岛素可刺激pp125FAK的去磷酸化；然而，其对桩蛋白的影响尚不清楚。我们发现胰岛素和胰岛素样生长因子-1（IGF-1）可刺激CHOT（过表达人胰岛素受体）细胞和CHOδCT69（过表达缺失C末端69个氨基酸的胰岛素受体）细胞中桩蛋白的去磷酸化。此外，在所使用的CHO（中国仓鼠卵巢）细胞系中，胰岛素或IGF-1刺激桩蛋白或pp125FAK去磷酸化并不需要胰岛素受体的C末端。
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本文引用的文献
1
Purified hybrid insulin/insulin-like growth factor-I receptors bind insulin-like growth factor-I, but not insulin, with high affinity.纯化的杂合胰岛素/胰岛素样生长因子-I受体以高亲和力结合胰岛素样生长因子-I，但不结合胰岛素。
Biochem J. 1993 Mar 1;290 ( Pt 2)(Pt 2):419-26. doi: 10.1042/bj2900419.
2
Tyrosine phosphorylation of the focal adhesion kinase pp125FAK during development: relation to paxillin.发育过程中粘着斑激酶pp125FAK的酪氨酸磷酸化：与桩蛋白的关系。
J Cell Sci. 1993 Jul;105 ( Pt 3):637-45. doi: 10.1242/jcs.105.3.637.
3
4
5
Role of the carboxyl-terminal domains of the insulin and insulin-like growth factor I receptors in receptor function.胰岛素及胰岛素样生长因子I受体的羧基末端结构域在受体功能中的作用。
J Biol Chem. 1994 May 13;269(19):13922-8.
6
Insulin receptor autophosphorylation and exogenous substrate phosphorylation: role of receptor C-terminus and effects of mild reduction.
Biochem Biophys Res Commun. 1994 Apr 15;200(1):330-7. doi: 10.1006/bbrc.1994.1452.
7
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J Biol Chem. 1995 Jan 20;270(3):991-4. doi: 10.1074/jbc.270.3.991.
8
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Endocr Rev. 1995 Apr;16(2):117-42. doi: 10.1210/edrv-16-2-117.
9
Signal transduction through integrins: a central role for focal adhesion kinase?整合素介导的信号转导：黏着斑激酶起核心作用？
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10
Activation of the small GTP-binding proteins rho and rac by growth factor receptors.生长因子受体对小GTP结合蛋白rho和rac的激活作用。
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