Brady J D, Sadler I H, Fry S C
Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh, Scotland, U.K.
Biochem J. 1996 Apr 1;315 ( Pt 1)(Pt 1):323-7. doi: 10.1042/bj3150323.
A novel amino acid, di-isodityrosine, has been isolated from hydrolysates of cell walls of tomato cell culture. Analysis by UV spectrometry, partial derivatization with 2,4-dinitrofluorobenzene and mass and NMR spectrometry show that the compound is composed to two molecules of isodityrosine, joined by a biphenyl linkage. The possible reactions involved in the formation of this molecule in vivo are discussed, as is the possibility that it could form an interpolypeptide linkage between cell wall proteins such as extensin, and hence aid in the insolubilization of the protein in the wall.
一种新型氨基酸——二异二酪氨酸,已从番茄细胞培养液细胞壁的水解产物中分离出来。通过紫外光谱分析、用2,4-二硝基氟苯进行部分衍生化以及质谱和核磁共振光谱分析表明,该化合物由两个异二酪氨酸分子组成,通过联苯键相连。文中讨论了该分子在体内形成过程中可能涉及的反应,以及它在细胞壁蛋白(如伸展蛋白)之间形成多肽间连接从而有助于细胞壁中蛋白质不溶性形成的可能性。