Soto F, Garcia-Guzman M, Karschin C, Stühmer W
Department of Molecular Biology of Neuronal Signals, Max-Planck Institute for Experimental Medicine, Göttingen, Germany.
Biochem Biophys Res Commun. 1996 Jun 14;223(2):456-60. doi: 10.1006/bbrc.1996.0915.
We have isolated the cDNA for a novel member (P2X6) of the ATP-gated ion channel family. The rat P2X6 nucleotide sequence encodes a 379 amino acid protein that conserves all the structural features of previously cloned P2X receptors, including the two putative transmembrane domains predicted by hydrophobicity plots. In situ hybridization analysis of rat brain sections showed a wide pattern of mRNA expression that is virtually identical to that already described for P2X4. Injection of P2X6 cRNA in Xenopus oocytes did not give rise to ATP-activated channels. Coexpression of P2X6 with P2X4 subunits produced currents which were not discernibly different from those of P2X4 expressed alone.
我们已经分离出一种ATP门控离子通道家族新成员（P2X6）的cDNA。大鼠P2X6核苷酸序列编码一种379个氨基酸的蛋白质，该蛋白质保留了先前克隆的P2X受体的所有结构特征，包括通过疏水性图谱预测的两个假定跨膜结构域。对大鼠脑切片的原位杂交分析显示，mRNA表达模式广泛，与已报道的P2X4的表达模式几乎相同。将P2X6的cRNA注射到非洲爪蟾卵母细胞中未产生ATP激活的通道。P2X6与P2X4亚基共表达产生的电流与单独表达的P2X4产生的电流没有明显差异。
