Nandy A
Computer Division, Indian Institute of Chemical Biology, Calcutta, India.
Comput Appl Biosci. 1996 Feb;12(1):55-62. doi: 10.1093/bioinformatics/12.1.55.
We have shown earlier that analysis of DNA sequences using a two-dimensional graphical representation provides considerable information on new global sequence patterns and homologies, repeated structures, relative base abundances, probable evolutionary paths and evolutionary divergence. We have also reported that at a more micro level the graphical representation reveals distinct differences in the features of intron and exon segments of eukaryotic sequences. In this paper, the distinguishing features of the intron and exon segments are exploited to show, through several examples of different gene structures, that an averaging procedure over the slopes of the representative maps provides an easy technique to differentiate between probable intron and exon regions. We thus expect that this method will enable a rapid search and preliminary indication of possible locations of protein coding regions in long eukaryotic sequences.
我们之前已经表明，使用二维图形表示法对DNA序列进行分析可提供有关新的全局序列模式和同源性、重复结构、相对碱基丰度、可能的进化路径和进化分歧的大量信息。我们还报告过，在更微观的层面上，图形表示法揭示了真核生物序列内含子和外显子片段特征的明显差异。在本文中，利用内含子和外显子片段的显著特征，通过几个不同基因结构的例子表明，对代表性图谱斜率进行平均的程序提供了一种区分可能的内含子和外显子区域的简便技术。因此，我们期望这种方法能够在长真核生物序列中快速搜索并初步指示蛋白质编码区域的可能位置。
