Braun F, Hajnsdorf E, Régnier P
Institut de Biologie Physico-Chimique, Paris, France.
Mol Microbiol. 1996 Mar;19(5):997-1005. doi: 10.1046/j.1365-2958.1996.440971.x.
The monocistronic transcript of rpsO undergoes an endonucleolytic cleavage downstream of the coding sequence, which removes the hairpin of the transcription terminator and initiates the rapid degradation of the message. We demonstrate here that the two rne-dependent cleavages, on both sides of the transcription terminator, are catalysed by RNase E in vitro and that the RNase E-processed rpsO message is rapidly degraded by polynucleotide phosphorylase, while RNase II produces stable decay intermediates. Moreover, we show that RNase E cuts in vitro the coding sequence of the rpsO mRNA at several sites which are not detected in vivo.
rpsO的单顺反子转录本在编码序列下游发生内切核酸酶切割，这会去除转录终止子的发夹结构并启动该信使RNA的快速降解。我们在此证明，转录终止子两侧的两个依赖于RNase E的切割在体外由RNase E催化，并且经RNase E处理的rpsO信使RNA被多核苷酸磷酸化酶快速降解，而RNase II产生稳定的降解中间体。此外，我们表明RNase E在体外切割rpsO mRNA的编码序列，在体内未检测到的几个位点进行切割。
