Palanichelvam K, Veluthambi K
Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai-625 021, India
Curr Microbiol. 1996 Sep;33(3):156-62. doi: 10.1007/s002849900093.
Octopine induced the synthesis of 83, 76, 62, 58, 44, 42, 31, and 22 kDa proteins in Agrobacterium tumefaciens strains harboring the tumor-inducing (Ti) plasmids pTiA6 and pTiAch5. Nopaline induced the synthesis of 83, 76, 62, 58, 56, 44, 42, 31, and 22 kDa proteins in A. tumefaciens strains harboring the Ti plasmids pTiC58 and pTiT37. The molecular masses of proteins induced by octopine and nopaline were very similar. In accordance with the 'opine concept', octopine and nopaline were found to induce protein synthesis only in strains harboring the respective Ti plasmids. Arginine, a common catabolic product of octopine and nopaline, induced the synthesis of most of the proteins induced by the two opines. Our results show that only the initial step(s) of octopine and nopaline catabolism are induced by specific opines in the respective strains. The subsequent steps are likely to be regulated by arginine in both strains.
章鱼碱在携带致瘤（Ti）质粒pTiA6和pTiAch5的根癌土壤杆菌菌株中诱导了83、76、62、58、44、42、31和22 kDa蛋白质的合成。胭脂碱在携带Ti质粒pTiC58和pTiT37的根癌土壤杆菌菌株中诱导了83、76、62、58、56、44、42、31和22 kDa蛋白质的合成。章鱼碱和胭脂碱诱导产生的蛋白质的分子量非常相似。根据“冠瘿碱概念”，发现章鱼碱和胭脂碱仅在携带相应Ti质粒的菌株中诱导蛋白质合成。章鱼碱和胭脂碱的共同分解代谢产物精氨酸诱导了这两种冠瘿碱诱导产生的大多数蛋白质的合成。我们的结果表明，在各自的菌株中，只有章鱼碱和胭脂碱分解代谢的初始步骤是由特定的冠瘿碱诱导的。在这两种菌株中，后续步骤可能受精氨酸调控。
