人血清素受体亚型2（HTR2）、酯酶D（ESD）和fms相关酪氨酸激酶（FLT）同源物在牛12号染色体上的同线定位。

Syntenic assignment of human serotonin receptor subtype 2 (HTR2), esterase D (ESD), and fms-related tyrosine kinase (FLT) homologs to bovine chromosome 12.

作者信息

Sun H S, Womack J E, Kirkpatrick B W

机构信息

Department of Meat and Animal Science, University of Wisconsin-Madison, 1675 Observatory Drive, Madison, Wisconsin 53706, USA.

出版信息

Mamm Genome. 1996 Jul;7(7):518-9. doi: 10.1007/s003359900154.

DOI:10.1007/s003359900154

PMID:8672130

Abstract

Bovine x rodent somatic hybrid cells have been used to syntenically map three bovine genes homologous to loci on human Chromosome (Chr) 13. These three loci, fms-related tyrosine kinase gene (FLT), esterase D (ESD), and 5-hydroxytryptamine receptor 2 (HTR2; serotonin receptor subtype 2), were assigned to bovine Chr 12 (BTA12) with 91-95% concordance to the coagulation factor 10 (F10) locus. Along with a previously mapped BTA12 gene, retinoblastoma-1 (RB1), this conserved synteny group spans 178 cM on human Chr 13 (HSA13). Previous reports suggested homology between HSA13 and both BTA2 and BTA12. Results reported here extend the boundary of the HSA13-BTA12 comparative map, contradict the previous preliminary assignment of ESD to BTA2, and suggest instead that the q arm of HSA13 may be entirely conserved in BTA12.

摘要

牛与啮齿动物的体细胞杂种细胞已被用于对三个与人类13号染色体（Chr）上的基因座同源的牛基因进行同线定位。这三个基因座，即fms相关酪氨酸激酶基因（FLT）、酯酶D（ESD）和5-羟色胺受体2（HTR2；血清素受体亚型2），被定位到牛的12号染色体（BTA12）上，与凝血因子10（F10）基因座的一致性为91-95%。连同先前定位的BTA12基因视网膜母细胞瘤1（RB1），这个保守的同线群在人类13号染色体（HSA13）上跨度为178厘摩。先前的报道表明HSA13与BTA2和BTA12之间存在同源性。此处报道的结果扩展了HSA13-BTA12比较图谱的边界，与先前将ESD初步定位到BTA2的结果相矛盾，相反，这表明HSA13的q臂在BTA12中可能是完全保守的。