Sun H S, Womack J E, Kirkpatrick B W
Department of Animal Sciences, University of Wisconsin, Madison, California 53706, USA.
Anim Genet. 1996 Dec;27(6):421-2. doi: 10.1111/j.1365-2052.1996.tb00509.x.
Heterologous primers were used to amplify an exon and intron-containing segment of the bovine homologue of the human dopachrome tautomerase gene. After confirmation of homology by sequence analysis (exon sequence similarity greater than 90%), bovine-specific primers were developed for synteny mapping purposes. The dopachrome tautomerase gene was assigned to bovine chromosome 12 (BTA12) with 97% concordance to the coagulation factor 10 locus. Together with previous synteny mapping of bovine chromosome 12 genes, fms-related tyrosine kinase, esterase D and 5-hydroxytryptamine receptor 2, this assignment further indicates conservation between human chromosome 13q and bovine chromosome 12.
使用异源引物扩增人多巴色素互变异构酶基因的牛同源基因中一个包含外显子和内含子的片段。通过序列分析确认同源性（外显子序列相似性大于90%）后，为了进行同线性图谱绘制，设计了牛特异性引物。多巴色素互变异构酶基因被定位到牛的12号染色体（BTA12）上，与凝血因子10位点的一致性为97%。结合之前对牛12号染色体上基因（fms相关酪氨酸激酶、酯酶D和5-羟色胺受体2）的同线性图谱绘制，这一基因定位进一步表明人13号染色体q臂与牛12号染色体之间存在保守性。
