Rettenberger G, Klett C, Zechner U, Kunz J, Vogel W, Hameister H
Abteilung Medizinische Genetik, Universität Ulm, Germany.
Genomics. 1995 Mar 20;26(2):372-8. doi: 10.1016/0888-7543(95)80222-8.
By comparative gene mapping, extended conservation of synteny between different mammalian species has become apparent. Mapping in these species could be accelerated by exact visualization of the chromosomal segments that exhibit conserved synteny. We have hybridized human chromosome-specific DNA libraries onto porcine metaphase spreads to examine the extent of conservation of synteny between the two species. The hybridization signals on pig chromosomes are of variable quality, but the analysis allowed us to assign for all human autosomes homologous chromosomal segments in the pig karyotype. Extended conservation of synteny was observed, often comprising whole chromosomes. In our analysis 47 segments of conserved synteny common to the human and pig karyotype were identified. Intrachromosomal rearrangements by inversion within and between these segments are described. These rearrangements are common events during evolution. Our analysis shows that conservation of synteny between human and pig is three times more than between humans and mice and, consequently, is characterized by fewer, but larger conserved segments.
通过比较基因图谱分析，不同哺乳动物物种之间同线性的广泛保守性已变得明显。通过精确可视化显示保守同线性的染色体片段，可以加快在这些物种中的图谱绘制。我们已将人类染色体特异性DNA文库与猪中期染色体铺展物进行杂交，以研究这两个物种之间同线性的保守程度。猪染色体上的杂交信号质量不一，但该分析使我们能够在猪核型中为所有人类常染色体确定同源染色体片段。观察到同线性的广泛保守性，通常涉及整条染色体。在我们的分析中，鉴定出了人类和猪核型共有的47个保守同线性片段。描述了这些片段内部和之间通过倒位发生的染色体内重排。这些重排在进化过程中是常见事件。我们的分析表明，人与猪之间同线性的保守程度是人与小鼠之间的三倍，因此，其特点是保守片段数量更少，但片段更大。
