Nozaki M, Onishi Y, Togashi S, Miyamoto H
Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
DNA Cell Biol. 1996 Jun;15(6):505-9. doi: 10.1089/dna.1996.15.505.
To study the general physiological role of the Mo25 gene, which has been cloned from mouse cleavage-stage embryos, we isolated a Drosophila equivalent, dMo25, cDNA from an embryo cDNA library. The 2,222 nucleotides contained a single open reading frame encoding a polypeptide of 339 amino acid residues with a calculated molecular mass of 39,278 daltons. The deduced amino acid sequence of the dMo25 cDNA had 69.3% identity with mouse Mo25. A homology search revealed that these were similar to a protein encoded in an open reading frame near the calcineurin B subunit gene on chromosome XI in Saccharomyces cerevisiae. In particular, the carboxy-terminal region was highly conserved in Drosophila, mouse, and yeast. The dMo25 gene was mapped to the left arm of the third chromosome at 73AB, and 2.3- and 1.8-kb mRNA bands were detected during development and in adult Drosophila. Conservation of the gene structure and the wide expression profile indicated that the function of the gene is likely to be fundamental in many cell types as well as during development.
为了研究从小鼠卵裂期胚胎中克隆出的Mo25基因的一般生理作用，我们从胚胎cDNA文库中分离出了果蝇的同源物dMo25 cDNA。该2222个核苷酸包含一个单一的开放阅读框，编码一个由339个氨基酸残基组成的多肽，计算分子量为39278道尔顿。dMo25 cDNA推导的氨基酸序列与小鼠Mo25有69.3%的同一性。同源性搜索显示，它们与酿酒酵母第十一染色体上钙调神经磷酸酶B亚基基因附近的一个开放阅读框中编码的蛋白质相似。特别是，果蝇、小鼠和酵母的羧基末端区域高度保守。dMo25基因定位于第三染色体左臂的73AB处，在果蝇发育过程中和成虫中检测到2.3 kb和1.8 kb的mRNA条带。基因结构的保守性和广泛的表达谱表明，该基因的功能在许多细胞类型以及发育过程中可能是基本的。
