Kobayashi H, Morozumi T, Miyamoto C, Shimizu M, Yamada S, Ohashi S, Kubo M, Kimura K, Mitani K, Ito N, Yamamoto K
National Institute of Animal Health, Ibaraki, Japan.
J Vet Med Sci. 1996 Feb;58(2):109-13. doi: 10.1292/jvms.58.109.
The infection levels of Mycoplasma hyorhinis, M. hyopneumoniae and M. hyosynoviae in the lung of piglets were examined in relation to porcine reproductive and respiratory syndrome (PRRS). These animals consisted of 43 PRRS piglets with PRRS, 2 piglets infected with PRRS virus but symptom-free, and 10 control piglets free of PRRS virus and its antibody. M. hyorhinis was isolated from 40 of the 43 PRRS piglets, from 1 of the 2 latent infected piglets and from 3 of the 10 control piglets. The number of M. hyorhinis isolated from the lungs of PRRS piglets was more than 10(5) CFU/g, but those isolated from the latent infected piglets and the control piglets were less than 10(3) CFU/g. In addition to this, Haemophilus parasuis and Pasteurella spp. were frequently isolated from the piglets with PRRS (51.2% and 25.6%, respectively). On the other hand, M. hyopneumoniae was isolated from only 4 of 55 piglets tested, and M. hyosynoviae was not isolated. M. hyorhinis was also detected directly in the lung emulsion samples from almost all the PRRS piglets using a polymerase chain reaction-based method.
研究了猪繁殖与呼吸综合征（PRRS）与仔猪肺中猪鼻支原体、猪肺炎支原体和猪滑膜支原体感染水平之间的关系。这些动物包括43头患有PRRS的仔猪、2头感染PRRS病毒但无症状的仔猪以及10头无PRRS病毒及其抗体的对照仔猪。从43头PRRS仔猪中的40头、2头潜伏感染仔猪中的1头以及10头对照仔猪中的3头分离出了猪鼻支原体。从PRRS仔猪肺中分离出的猪鼻支原体数量超过10(5) CFU/g，但从潜伏感染仔猪和对照仔猪中分离出的数量少于10(3) CFU/g。此外，副猪嗜血杆菌和巴氏杆菌属也经常从患有PRRS的仔猪中分离出来（分别为51.2%和25.6%）。另一方面，在55头受试仔猪中仅从4头分离出了猪肺炎支原体，未分离出猪滑膜支原体。使用基于聚合酶链反应的方法，几乎在所有PRRS仔猪的肺乳剂样本中也直接检测到了猪鼻支原体。
