Olde Damink L H, Dijkstra P J, van Luyn M J, van Wachem P B, Nieuwenhuis P, Feijen J
Department of Chemical Technology, University of Twente, Enschede, The Netherlands.
Biomaterials. 1996 Apr;17(7):679-84. doi: 10.1016/0142-9612(96)86737-8.
Bacterial collagenase was used to study the susceptibility of dermal sheep collagen (DSC) cross-linked with a mixture of the water-soluble carbodiimide 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide hydrochloride and N-hydroxysuccinimide (E/N-DSC) towards enzymatic degradation. Contrary to non-cross-linked DSC (N-DSC), which had a rate of weight-loss of 18.1% per hour upon degradation, no weight loss was observed for E/N-DSC during a 24 h degradation period. The tensile strength of the E/N-DSC samples decreased during this time period, resulting in partially degraded samples having 80% of the initial tensile strength remaining. The susceptibility of E/N-DSC samples towards enzymatic degradation could be controlled by varying the degree of cross-linking of the samples. Ethylene oxide sterilization of E/N-DSC samples made the material more resistant against degradation compared with non-sterilized E/N-DSC samples. This may be explained by a decrease of the adsorption of bacterial collagenase onto the collagen owing to reaction of ethylene oxide with remaining free amine groups in the collagen matrix.
使用细菌胶原酶研究了用1-乙基-3-(3-二甲基氨基丙基)-碳二亚胺盐酸盐和N-羟基琥珀酰亚胺（E/N）混合物交联的绵羊真皮胶原蛋白（DSC）对酶促降解的敏感性。与未交联的DSC（N-DSC）不同，N-DSC在降解时每小时的失重率为18.1%，而在24小时的降解期内，E/N-DSC未观察到失重。在此时间段内，E/N-DSC样品的拉伸强度下降，导致部分降解的样品仍保留80%的初始拉伸强度。E/N-DSC样品对酶促降解的敏感性可通过改变样品的交联程度来控制。与未灭菌的E/N-DSC样品相比，E/N-DSC样品的环氧乙烷灭菌使材料更耐降解。这可能是由于环氧乙烷与胶原蛋白基质中剩余的游离胺基反应，导致细菌胶原酶在胶原蛋白上的吸附减少所致。
