[卵胞浆内单精子注射中的形态学]
[Morphology in intracytoplasmic sperm injection].
作者信息
Küpker W, al-Hasani S, Schulze W, Kühnel W, Schill T, Felberbaum R, Diedrich K
机构信息
Universitäts-Frauenklinik, Medizinischen Universität Lübeck.
出版信息
Gynakol Geburtshilfliche Rundsch. 1995;35 Suppl 1:132-6. doi: 10.1159/000272584.
OBJECTIVE
Was to determine if severe morphological defects of spermatozoa in Oligo-Astheno-Teratozoospermia (OAT) have any impact on the fertilization process in intracytoplasmic sperm injection (ICSI) and, if there are any specific morphological characteristics of unfertilized oocytes after the ICSI procedure.
METHODS
Ejaculates of males with severe subfertility and unfertilized oocytes after the ICSI procedure were investigated by transmissionelectronmicroscopy.
RESULTS
ICSI provided a fertilization rate of 66% in cases of severe defective spermatozoa. The ultrastructure revealed severe head and neck alterations in particular. The ultrastructure of the unfertilized oocyte displayed mostly no signs of activation and lack of decondensation of sperm chromatin.
CONCLUSIONS
Our findings support the hypothesis that not severe sperm defects but the lack of intracytoplasmic sperm-oocyte interaction displays the most critical role in the fertilization process in intracytoplasmic sperm injection.
目的
确定少弱畸精子症（OAT）中精子的严重形态缺陷是否对卵胞浆内单精子注射（ICSI）的受精过程有任何影响，以及ICSI术后未受精卵子是否有任何特定的形态特征。
方法
通过透射电子显微镜对严重生育力低下男性的射精样本以及ICSI术后未受精的卵子进行研究。
结果
在精子严重缺陷的情况下，ICSI的受精率为66%。超微结构显示特别是头部和颈部有严重改变。未受精卵子的超微结构大多没有激活迹象，精子染色质也没有解聚。
结论
我们的研究结果支持这一假设，即在卵胞浆内单精子注射的受精过程中，不是严重的精子缺陷，而是缺乏胞浆内精子 - 卵子相互作用起着最关键的作用。
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