[免疫调节剂对绒毛膜癌细胞的修饰。细胞因子与绒毛膜癌细胞]
[Modification of chorionic carcinoma cells by immunomodulators. Cytokines and chorionic carcinoma cells].
作者信息
Marth C, Berger P, Zwierzina H, Daxenbichler G
机构信息
Univ. Klinik für Frauenheilkunde, Innsbruck.
出版信息
Gynakol Geburtshilfliche Rundsch. 1995;35 Suppl 1:9-10. doi: 10.1159/000272554.
OBJECTIVES
The role of inflammatory cytokines on regulation of hCG biosynthesis was studied.
METHODS
JAR choriocarcinoma cells were cultured and treated with interleukin-1 (IL-1), IL-3. IL-4, stem cell factor (SCF), tumor necrosis factor (TNF), and granulocyte or granulocyte-macrophage-colony stimulating factor. hCG concentration was determined by immunoradiometric methods and cell number was enumerated by means of an electronic particle counter.
RESULTS
Il-1 and TNF increased biosynthesis of holo-hCG as well as the free alpha- and free beta-subunit and the beta-core protein. Proliferation was not affected by any cytokine tested.
CONCLUSION
Inflammatory cytokines are able to increase the biosynthesis of hCG.
目的
研究炎性细胞因子对人绒毛膜促性腺激素（hCG）生物合成调节的作用。
方法
培养JAR绒毛膜癌细胞，并用白细胞介素-1（IL-1）、IL-3、IL-4、干细胞因子（SCF）、肿瘤坏死因子（TNF）以及粒细胞或粒细胞-巨噬细胞集落刺激因子进行处理。采用免疫放射分析方法测定hCG浓度，通过电子粒子计数器计数细胞数量。
结果
IL-1和TNF增加了全hCG以及游离α亚基、游离β亚基和β核心蛋白的生物合成。所测试的任何细胞因子均未影响细胞增殖。
结论
炎性细胞因子能够增加hCG的生物合成。
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