Christensen A H, Quail P H
Dept. of Plant Biology, University of California, Berkeley 94720, USA.
Transgenic Res. 1996 May;5(3):213-8. doi: 10.1007/BF01969712.
A set of plasmids has been constructed utilizing the promoter, 5' untranslated exon, and first intron of the maize ubiquitin (Ubi-1) gene to drive expression of protein coding sequences of choice. Plasmids containing chimaeric genes for ubiquitin-luciferase (Ubi-Luc), ubiquitin-beta-glucuronidase (Ubi-GUS), and ubiquitin-phosphinothricin acetyl transferase (Ubi-bar) have been generated, as well as a construct containing chimaeric genes for both Ubi-GUS and Ubi-bar in a single plasmid. Another construct was generated to allow cloning of protein coding sequences of choice on Bam HI and Bam HI-compatible restriction fragments downstream of the Ubi-1 gene fragment. Because the Ubi-1 promoter has been shown to be highly active in monocots, these constructs may be useful for generating high-level gene expression of selectable markers to facilitate efficient transformation of monocots, to drive expression of reference reporter genes in studies of gene expression, and to provide expression of biotechnologically important protein products in transgenic plants.
利用玉米泛素（Ubi-1）基因的启动子、5'非翻译外显子和第一内含子构建了一组质粒，以驱动所选蛋白质编码序列的表达。已构建了含有泛素-荧光素酶（Ubi-Luc）、泛素-β-葡萄糖醛酸酶（Ubi-GUS）和泛素-膦丝菌素乙酰转移酶（Ubi-bar）嵌合基因的质粒，以及在单个质粒中含有Ubi-GUS和Ubi-bar嵌合基因的构建体。还构建了另一种构建体，以便在Ubi-1基因片段下游的Bam HI和Bam HI兼容限制片段上克隆所选蛋白质编码序列。由于Ubi-1启动子已被证明在单子叶植物中具有高活性，这些构建体可用于产生选择标记的高水平基因表达，以促进单子叶植物的高效转化，在基因表达研究中驱动参考报告基因的表达，并在转基因植物中提供具有生物技术重要性的蛋白质产物的表达。
