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鉴定、克隆和表征水稻中两个针灸损伤诱导启动子。

Identification, Cloning, and Characterization of Two Acupuncture-Injury-Inducing Promoters in Rice.

机构信息

School of Life Sciences, Hubei University, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Wuhan 430062, China.

Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Laboratory of Crop Molecular Breeding, Food Crops Institute, Hubei Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430064, China.

出版信息

Int J Mol Sci. 2024 Sep 30;25(19):10564. doi: 10.3390/ijms251910564.

DOI:10.3390/ijms251910564
PMID:39408894
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11476359/
Abstract

As an important global food crop, rice is damaged by a variety of piercing-sucking pests. Identifying a broad-spectrum promoter induced by the physical signal of sucking pests and applying it to transgenic breeding to mitigate the damage caused by different sucking pests will significantly improve the efficiency of our breeding. This study compared the transcriptome changes in two rice varieties under needle-wounding stress to investigate their differential responses to mechanical damage. The results showed that the insect-susceptible variety TN1 exhibited more differentially expressed genes (DEGs) and greater changes in expression levels after needle treatment, indicating a more active internal gene regulatory network. GO and KEGG enrichment analysis further revealed that TN1 not only exhibited changes in genes related to the extracellular environment, but also mobilized more genes associated with stress response and defense. By screening the differentially expressed genes, we identified two promoters (P1 and P2) with inducible expression characteristics in both the resistant and susceptible rice varieties. These promoters were able to effectively drive the expression of the insect resistance gene * and enhance the resistance of transgenic plants against the brown planthopper. This study provides promoter resources for the development of insect-resistant transgenic crops.

摘要

作为一种重要的全球粮食作物，水稻受到各种刺吸式害虫的侵害。鉴定一种广谱启动子，该启动子由刺吸式害虫的物理信号诱导，并将其应用于转基因育种以减轻不同刺吸式害虫的危害，将显著提高我们的育种效率。本研究比较了两种水稻品种在针状刺伤胁迫下的转录组变化，以研究它们对机械损伤的差异响应。结果表明，感虫品种 TN1 在针刺处理后表现出更多差异表达基因（DEGs）和更大的表达水平变化，表明其内部基因调控网络更为活跃。GO 和 KEGG 富集分析进一步表明，TN1 不仅表现出与细胞外环境相关基因的变化，还动员了更多与应激反应和防御相关的基因。通过筛选差异表达基因，我们在抗性和感病水稻品种中鉴定出两个具有诱导表达特性的启动子（P1 和 P2）。这些启动子能够有效地驱动抗虫基因的表达，并增强转基因植物对褐飞虱的抗性。本研究为开发抗虫转基因作物提供了启动子资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e0e/11476359/0fa23964976d/ijms-25-10564-g007.jpg
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