Gajkowska B, Mossakowski M J
Laboratory of the Ultrastructure of the Nervous System, Polish Academy of Sciences, Warszawa.
Folia Neuropathol. 1997;35(3):171-80.
Electron microscopy immunocytochemical study was performed to clarify ultrastructural localization and role of endothelial nitric oxide synthase (EC-NOS) in the endothelial cells (EC) of rat hippocampal vessels after transient cerebral ischemia. EC-NOS immunoreactivity was found in the endothelial cells in association with plasma membrane, sub-plasmalemmal vesicles, basal membrane and in cytosol (cytoplasm free of subcellular organelles). A sharp transient increase in immunoreactivity of NOS was observed at 10 min up to 1 hour after ischemia. The results of the present study indicate that NO, as a potent vasodilator, may play a protective role in ischemic brain damage.
进行电子显微镜免疫细胞化学研究，以阐明短暂性脑缺血后大鼠海马血管内皮细胞（EC）中内皮型一氧化氮合酶（EC-NOS）的超微结构定位及其作用。在与质膜、质膜下小泡、基底膜相关的内皮细胞以及胞质溶胶（不含亚细胞器的细胞质）中发现了EC-NOS免疫反应性。缺血后10分钟至1小时观察到NOS免疫反应性急剧短暂增加。本研究结果表明，作为一种强效血管舒张剂，NO可能在缺血性脑损伤中发挥保护作用。
