Gerli R, Muscat C, Bertotto A, Bistoni O, Agea E, Tognellini R, Fiorucci G, Cesarotti M, Bombardieri S
Institute of Internal Medicine, University of Perugia, Italy.
Clin Immunol Immunopathol. 1996 Jul;80(1):31-7. doi: 10.1006/clin.1996.0091.
T cell surface expression and the functional role of CD26 antigen (Ag), a surface ectoenzyme involved in T cell activation and migration across the extracellular matrix, were analyzed in the peripheral blood (PB) and synovial fluid (SF) from patients with inflammatory arthritides. CD26 membrane expression on T cells was detected by cytofluorometry using two different monoclonal antibodies, anti-Ta1 and anti-1F7, while cell proliferation and both IL-2 and IFN-gamma production were evaluated in anti-CD3- or anti-CD2-stimulated cell cultures after Ag surface modulation with anti-1F7. The results showed that Ta1 and 1F7 Ag expression were increased on T cells from PB of patients with active, but not inactive, rheumatoid arthritis (RA). Most SF T cells from RA or other inflammatory arthritides displayed the memory marker CD45R0 and the Ta1 Ag, but lacked the 1F7 molecule. In addition, in vitro 1F7 modulation, which enhanced RA PB T cell proliferation and both IL-2 and IFN-gamma synthesis, did not synergize with anti-CD3 or anti-CD2 in inducing IL-2-dependent activation of SF T cells, but reduced IFN-gamma production. A spontaneous reappearance of 1F7 Ag on the SF T cell surface was seen after 2-5 days in culture. Phorbol myristate acetate, able to accelerate its reexpression, also restored a normal response of SF T cells to anti-1F7 comitogenic effects. These data confirm a role of the CD26 surface molecule in regulating T cell activation and lymphokine synthesis. This observation may have important implications in the regulation of T cell activity at the joint level during chronic inflammatory processes.
对参与T细胞活化及穿越细胞外基质迁移的表面外切酶CD26抗原（Ag）在炎性关节炎患者外周血（PB）和滑液（SF）中的T细胞表面表达及功能作用进行了分析。使用两种不同的单克隆抗体抗-Ta1和抗-1F7，通过细胞荧光测定法检测T细胞上的CD26膜表达，而在用抗-1F7进行Ag表面调节后，在抗-CD3或抗-CD2刺激的细胞培养物中评估细胞增殖以及IL-2和IFN-γ的产生。结果显示，活动性类风湿关节炎（RA）患者PB中的T细胞上Ta1和1F7 Ag表达增加，而非活动性患者则不然。来自RA或其他炎性关节炎的大多数SF T细胞显示记忆标志物CD45R0和Ta1 Ag，但缺乏1F7分子。此外，体外1F7调节可增强RA PB T细胞增殖以及IL-2和IFN-γ合成，但在诱导SF T细胞的IL-2依赖性活化方面与抗-CD3或抗-CD2无协同作用，反而降低了IFN-γ的产生。培养2 - 5天后，SF T细胞表面出现1F7 Ag的自发再现。能够加速其重新表达的佛波醇肉豆蔻酸酯也恢复了SF T细胞对抗-1F7促有丝分裂作用的正常反应。这些数据证实了CD26表面分子在调节T细胞活化和淋巴因子合成中的作用。这一观察结果可能对慢性炎症过程中关节水平T细胞活性的调节具有重要意义。
