Mizokami A, Eguchi K, Kawakami A, Ida H, Kawabe Y, Tsukada T, Aoyagi T, Maeda K, Morimoto C, Nagataki S
First Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
J Rheumatol. 1996 Dec;23(12):2022-6.
To investigate the activation of T cells in peripheral blood (PB) and synovial fluid (SF) of patients with rheumatoid arthritis (RA).
The expression of CD26 (Ta1 and 1F7) antigen on T cells was analyzed in 7 women with RA and 7 healthy control subjects by immunofluorescence.
The percentage of CD3+ CD26+ cells was significantly higher in PB of patients with RA compared with healthy subjects. The IF7+ cell population was divided into high (1F7+high cells) and low fluorescence populations (1F7+low cells), based on 1F7 antigen density. The percentage of 1F7+high cells in SF of RA was markedly increased compared with PB of patients and healthy subjects. However, RA SF contained lower percentages of whole 1F7+ cells compared with PB.
Our results indicate that SF of patients with RA contains activated T cells, and suggest that T cells with high levels of CD26 antigen may preferentially migrate into the rheumatoid synovium to induce inflammation and tissue destruction.
研究类风湿关节炎（RA）患者外周血（PB）和滑液（SF）中T细胞的活化情况。
采用免疫荧光法分析7例RA女性患者和7例健康对照者T细胞上CD26（Ta1和1F7）抗原的表达。
与健康受试者相比，RA患者PB中CD3+CD26+细胞的百分比显著更高。根据1F7抗原密度，IF7+细胞群体分为高荧光群体（1F7+高细胞）和低荧光群体（1F7+低细胞）。与患者的PB和健康受试者相比，RA患者SF中1F7+高细胞的百分比显著增加。然而，与PB相比，RA患者SF中全1F7+细胞的百分比更低。
我们的结果表明，RA患者的SF中含有活化的T细胞，并提示CD26抗原水平高的T细胞可能优先迁移至类风湿滑膜以诱导炎症和组织破坏。
