血液储存XXIII：在含和不含亚甲蓝的CPD-腺嘌呤-肌苷保存液中2,3-二磷酸甘油酸维持六周
Blood storage XXIII: 2,3-DPG maintenance for six weeks in a CPD-adenine-inosine preservative with and without methylene blue.
作者信息
Dawson R B
出版信息
Transfusion. 1977 May-Jun;17(3):238-41. doi: 10.1046/j.1537-2995.1977.17377196358.x.
In a pilot study the optimal concentration of inosine for 2,3-DPG maintenance in a CPD-adenine (0.25 mM) preservative was confirmed to be at lease 10 mM. In these experiments, 2,3-DPG maintenance was nearly normal for six weeks of storage in CPD-adenine-inosine (10 mM) preservative with or without methylene blue. The control preservative lacking inosine showed a statistically significant decrease in 2,3-DPG concentrations after the 3rd week. Finally, 2,3-DPG levels were significantly better maintained in CPD-adenine preservatives that contained 15 mM concentrations of inosine, whether methylene blue was present or not (10(-6)M), compared to CPD-adenine-inosine preservatives that contained 5 mM inosine, with or without methylene blue. The methylene blue effect, while it can be demonstrated in most experiments to help the red blood cell maintain 2,3-DPG during prolonged blood storage, is judges to be a slight value. However, inosine is of great value in maintaining 2,3-DPG for prolonged (five to six weeks) liquid storage.
在一项初步研究中，已证实，在CPD-腺嘌呤（0.25 mM）防腐剂中，用于维持2,3-二磷酸甘油酸（2,3-DPG）的肌苷最佳浓度至少为10 mM。在这些实验中，在含有或不含有亚甲蓝的CPD-腺嘌呤-肌苷（10 mM）防腐剂中储存六周，2,3-DPG的维持情况几乎正常。缺乏肌苷的对照防腐剂在第3周后2,3-DPG浓度出现统计学显著下降。最后，与含有5 mM肌苷（含有或不含有亚甲蓝）的CPD-腺嘌呤-肌苷防腐剂相比，无论是否存在亚甲蓝（10⁻⁶M），含有15 mM肌苷的CPD-腺嘌呤防腐剂能更好地维持2,3-DPG水平。亚甲蓝的作用，虽然在大多数实验中可以证明其有助于红细胞在长时间血液储存期间维持2,3-DPG，但被认为价值不大。然而，肌苷对于长时间（五到六周）液体储存维持2,3-DPG具有重要价值。
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