Niederberger C, Fankhauser H, Edenharter E, Schweingruber M E
Institute of General Microbiology, University of Bern, Switzerland.
Gene. 1996 May 24;171(1):119-22. doi: 10.1016/0378-1119(96)00101-1.
Amiloride (Am) inhibits growth in the fission yeast Schizosaccharomyces pombe. We show that the toxic effect of this drug is relieved by low concentrations of thiamine (Th) and that the pyrimidine moiety of the Th molecule is responsible for growth inhibition release. A putative membrane protein encoded by the car1 gene is the target for Am action. It is responsible for Am sensitivity and is involved in the utilization of Th and its biosynthetic precursor, 4-amino-5-hydroxymethyl-2-methylpyrimidine. Its expression is repressed by Th and is under the genetic control of the genes, thi1, tnr1, tnr2 and tnr3, which have previously been shown to be responsible for the transcriptional control of genes involved in the biosynthesis and dephosphorylation of Th.
氨氯吡咪（Am）抑制裂殖酵母粟酒裂殖酵母的生长。我们发现，低浓度的硫胺素（Th）可缓解这种药物的毒性作用，且Th分子的嘧啶部分负责解除生长抑制。car1基因编码的一种假定膜蛋白是Am作用的靶点。它导致对Am敏感，并参与Th及其生物合成前体4-氨基-5-羟甲基-2-甲基嘧啶的利用。其表达受Th抑制，并且受thi1、tnr1、tnr2和tnr3基因的遗传控制，这些基因先前已被证明负责Th生物合成和去磷酸化相关基因的转录控制。
