通过密度梯度离心法对牛基因组进行分析：在硫酸铯/3,6-双（乙酰汞甲基）二恶烷密度梯度中的分级分离。
An analysis of the bovine genome by density gradient centrifugation: fractionation in Cs2SO4/3,6-bis(acetatomercurimethyl)dioxane density gradient.
作者信息
Cortadas J, Macaya G, Bernardi G
出版信息
Eur J Biochem. 1977 Jun 1;76(1):13-9. doi: 10.1111/j.1432-1033.1977.tb11565.x.
Abstract
The fractionation of calf thymus DNA by centrifugation in density gradients of Cs2SO4/BAMD, where BAMD = 3,6-bis(acetatomercurimethyl)dioxane, is described. A large-scale separation of (dG+dC)-rich DNA fractions has been obtained, allowing the relative amounts of minor and satellite components in the bovine genome to be precisely assessed.
摘要
描述了通过在Cs2SO4/BAMD（其中BAMD = 3,6-双（乙酰汞甲基）二恶烷）密度梯度中离心对小牛胸腺DNA进行分级分离的方法。已实现了富含（dG + dC）的DNA组分的大规模分离，从而能够精确评估牛基因组中次要成分和卫星成分的相对含量。
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