• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Effects of the apolipoprotein E polymorphism on uptake and transfer of cell-derived cholesterol in plasma.

作者信息

Huang Y, von Eckardstein A, Wu S, Assmann G

机构信息

Institut für Arterioskleroseforschung an der Universität Münster, Federal Republic of Germany.

出版信息

J Clin Invest. 1995 Dec;96(6):2693-701. doi: 10.1172/JCI118336.

DOI:10.1172/JCI118336
PMID:8675636
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC185976/
Abstract

The reverse cholesterol transport is initiated by the uptake of cholesterol into minor subfractions of high density lipoproteins (HDL) which contain either apolipoprotein (apo) A-I or apoE as their only apolipoproteins. From these initial acceptors, which are termed prebeta1-LpA-I and gamma-LpE, respectively, cell-derived cholesterol is transferred to LDL via the bulk of HDL termed alpha-LpA-I. In this study we analyzed the effect in plasma of the genetically determined apoE polymorphism on the formation of gamma-LpE, uptake and transfer of cell-derived cholesterol to LDL. Gamma-LpE was immunologically detectable in plasmas of individuals carrying at least one apoE3-allele but not in apoE3-free plasmas. During one minute incubation with [3H]cholesterol-labeled fibroblasts, gamma-LpE of plasmas from apoE3/3 subjects accumulated 7 and 13-fold more radioactivity than the respective fractions in plasmas from apoE2/2- and apoE4/4-subjects, respectively. Totally, 30% less [3H]cholesterol was released into plasmas of apoE2/2 and apoE4/4-individuals as compared with plasmas of apoE3/3-subjects. Moreover, plasmas of apoE3/3 individuals accumulated 50% and 65% more cell-derived [3H]cholesterol in alpha-LpA-I2 than plasmas of apoE4/4 and apoE2/2-subjects, respectively. These results indicate that the apoE-polymorphism is an important determinant of the uptake and transfer of cell-derived cholesterol in plasma.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/2c0f69f85abf/jcinvest00018-0162-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/9c099e33115e/jcinvest00018-0161-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/5b3b1ea0d868/jcinvest00018-0161-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/6ef68ec62f00/jcinvest00018-0161-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/6466155e0417/jcinvest00018-0161-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/f7d9abe36b5e/jcinvest00018-0161-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/121e93b79cf2/jcinvest00018-0161-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/b16211e16992/jcinvest00018-0162-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/67c5136dd4d8/jcinvest00018-0162-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/c18d63b6a2a3/jcinvest00018-0162-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/2c0f69f85abf/jcinvest00018-0162-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/9c099e33115e/jcinvest00018-0161-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/5b3b1ea0d868/jcinvest00018-0161-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/6ef68ec62f00/jcinvest00018-0161-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/6466155e0417/jcinvest00018-0161-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/f7d9abe36b5e/jcinvest00018-0161-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/121e93b79cf2/jcinvest00018-0161-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/b16211e16992/jcinvest00018-0162-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/67c5136dd4d8/jcinvest00018-0162-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/c18d63b6a2a3/jcinvest00018-0162-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a61b/185976/2c0f69f85abf/jcinvest00018-0162-d.jpg

相似文献

1
Effects of the apolipoprotein E polymorphism on uptake and transfer of cell-derived cholesterol in plasma.
J Clin Invest. 1995 Dec;96(6):2693-701. doi: 10.1172/JCI118336.
2
Cholesterol efflux from normal and Tangier disease fibroblasts into normal, high-density lipoprotein-deficient, and apolipoprotein E-deficient plasmas.胆固醇从正常和丹吉尔病成纤维细胞外流至正常、高密度脂蛋白缺乏及载脂蛋白E缺乏的血浆中。
Metabolism. 2000 Jun;49(6):770-7. doi: 10.1053/meta.2000.6243.
3
The effect of apoE genotype and sex on ApoE plasma concentration is determined by dietary fat in healthy subjects.在健康受试者中，载脂蛋白E（ApoE）基因型和性别对血浆ApoE浓度的影响由饮食脂肪决定。
Br J Nutr. 2009 Jun;101(12):1745-52. doi: 10.1017/S0007114508111515. Epub 2008 Nov 24.
4
Reverse cholesterol transport in plasma of patients with different forms of familial HDL deficiency.不同类型家族性高密度脂蛋白缺乏症患者血浆中的胆固醇逆向转运
Arterioscler Thromb Vasc Biol. 1995 May;15(5):691-703. doi: 10.1161/01.atv.15.5.691.
5
[Relationship between gene polymorphism at the apolipoprotein E locus and serum lipid profile in urban children of school age in Beijing].[北京城区学龄儿童载脂蛋白E基因多态性与血脂谱的关系]
Zhonghua Yu Fang Yi Xue Za Zhi. 2001 Sep;35(5):297-300.
6
Phospholipid transfer protein mediated conversion of high density lipoproteins generates pre beta 1-HDL.磷脂转运蛋白介导的高密度脂蛋白转化产生前β1-高密度脂蛋白。
Biochim Biophys Acta. 1996 Jun 11;1301(3):255-62. doi: 10.1016/0005-2760(96)00050-1.
7
[Effects of polymorphism of apolipoprotein E gene on body mass index and plasma lipid levels in obese children].载脂蛋白E基因多态性对肥胖儿童体重指数及血脂水平的影响
Zhonghua Yu Fang Yi Xue Za Zhi. 2001 Nov;35(6):412-4.
8
Abnormal in vivo metabolism of apolipoprotein E4 in humans.人类载脂蛋白E4的体内代谢异常。
J Clin Invest. 1986 Sep;78(3):815-21. doi: 10.1172/JCI112645.
9
Interaction of nascent ApoE2, ApoE3, and ApoE4 isoforms expressed in mammalian cells with amyloid peptide beta (1-40). Relevance to Alzheimer's disease.哺乳动物细胞中表达的新生载脂蛋白E2、载脂蛋白E3和载脂蛋白E4亚型与β淀粉样肽（1-40）的相互作用。与阿尔茨海默病的相关性。
Biochemistry. 1997 Aug 26;36(34):10571-80. doi: 10.1021/bi9626362.
10
[Relationship of APOE gene polymorphism with subclasses of serum high density lipoprotein in hyperlipidemia].[载脂蛋白E基因多态性与高脂血症患者血清高密度脂蛋白亚类的关系]
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2004 Dec;21(6):615-8.

引用本文的文献

1
Cholesteryl Ester Transfer Protein Deficiency and Hyperalphalipoproteinemia.胆固醇酯转运蛋白缺乏症与高α脂蛋白血症。
J Atheroscler Thromb. 2025 Aug 1;32(8):911-923. doi: 10.5551/jat.RV22037. Epub 2025 May 29.
2
Cholesterol and matrisome pathways dysregulated in astrocytes and microglia.胆固醇和基质体途径在星形胶质细胞和小胶质细胞中失调。
Cell. 2022 Jun 23;185(13):2213-2233.e25. doi: 10.1016/j.cell.2022.05.017.
3
Differential associations between apolipoprotein E alleles and cerebral myelin content in normative aging.载脂蛋白 E 等位基因与正常衰老过程中脑髓鞘含量的差异关联。

本文引用的文献

1
A simple method for the isolation and purification of total lipides from animal tissues.一种从动物组织中分离和纯化总脂质的简单方法。
J Biol Chem. 1957 May;226(1):497-509.
2
Quantitation of pre beta-HDL-dependent and nonspecific components of the total efflux of cellular cholesterol and phospholipid.细胞胆固醇和磷脂总流出中前β-HDL依赖性和非特异性成分的定量分析。
Biochemistry. 1993 May 18;32(19):5025-8. doi: 10.1021/bi00070a008.
3
Cell-derived unesterified cholesterol cycles between different HDLs and LDL for its effective esterification in plasma.
Neuroimage. 2022 May 1;251:118988. doi: 10.1016/j.neuroimage.2022.118988. Epub 2022 Feb 9.
4
ApoE and ApoE Nascent-Like HDL Particles at Model Cellular Membranes: Effect of Protein Isoform and Membrane Composition.载脂蛋白E及模型细胞膜上类似新生高密度脂蛋白的载脂蛋白E颗粒：蛋白质异构体和膜组成的影响
Front Chem. 2021 Apr 29;9:630152. doi: 10.3389/fchem.2021.630152. eCollection 2021.
5
Differential Effects of apoE4 and Activation of ABCA1 on Brain and Plasma Lipoproteins.载脂蛋白E4及ATP结合盒转运体A1激活对脑和血浆脂蛋白的不同影响。
PLoS One. 2016 Nov 8;11(11):e0166195. doi: 10.1371/journal.pone.0166195. eCollection 2016.
6
Human plasma lipid modulation in schistosomiasis mansoni depends on apolipoprotein E polymorphism.曼氏血吸虫病患者的人体血浆脂质调节取决于载脂蛋白E多态性。
PLoS One. 2014 Jul 22;9(7):e101964. doi: 10.1371/journal.pone.0101964. eCollection 2014.
7
Interaction between VLDL and phosphatidylcholine liposomes generates new γ-LpE-like particles.极低密度脂蛋白（VLDL）与磷脂酰胆碱脂质体之间的相互作用产生了新的γ-脂蛋白E样颗粒。
Lipids. 2014 Feb;49(2):143-53. doi: 10.1007/s11745-013-3861-8.
8
Apolipoprotein E gene polymorphism influences aggressive behavior in prostate cancer cells by deregulating cholesterol homeostasis.载脂蛋白 E 基因多态性通过扰乱胆固醇稳态影响前列腺癌细胞的侵袭行为。
Int J Oncol. 2013 Oct;43(4):1002-10. doi: 10.3892/ijo.2013.2057. Epub 2013 Aug 7.
9
Targeted In Situ Gene Correction of Dysfunctional APOE Alleles to Produce Atheroprotective Plasma ApoE3 Protein.靶向纠正功能失调的 APOE 等位基因以产生抗动脉粥样硬化的血浆 ApoE3 蛋白。
Cardiol Res Pract. 2012;2012:148796. doi: 10.1155/2012/148796. Epub 2012 May 7.
10
Apolipoprotein E and cholesterol in aging and disease in the brain.载脂蛋白E与大脑衰老及疾病中的胆固醇
Future Lipidol. 2008 Oct;3(5):505-530. doi: 10.2217/17460875.3.5.505.
细胞来源的未酯化胆固醇在不同的高密度脂蛋白（HDL）和低密度脂蛋白（LDL）之间循环，以便在血浆中进行有效的酯化。
Arterioscler Thromb. 1993 Mar;13(3):445-58. doi: 10.1161/01.atv.13.3.445.
4
Relation of apolipoprotein E phenotype to myocardial infarction and mortality from coronary artery disease.
Am J Cardiol. 1993 Jan 15;71(2):160-5. doi: 10.1016/0002-9149(93)90732-r.
5
The two-receptor model of lipoprotein clearance: tests of the hypothesis in "knockout" mice lacking the low density lipoprotein receptor, apolipoprotein E, or both proteins.脂蛋白清除的双受体模型：在缺乏低密度脂蛋白受体、载脂蛋白E或这两种蛋白的“基因敲除”小鼠中对该假说的验证。
Proc Natl Acad Sci U S A. 1994 May 10;91(10):4431-5. doi: 10.1073/pnas.91.10.4431.
6
A plasma lipoprotein containing only apolipoprotein E and with gamma mobility on electrophoresis releases cholesterol from cells.一种仅含载脂蛋白E且在电泳时具有γ迁移率的血浆脂蛋白可从细胞中释放胆固醇。
Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1834-8. doi: 10.1073/pnas.91.5.1834.
7
A cell culture system for screening human serum for ability to promote cellular cholesterol efflux. Relations between serum components and efflux, esterification, and transfer.一种用于筛选人血清促进细胞胆固醇流出能力的细胞培养系统。血清成分与流出、酯化及转运之间的关系。
Arterioscler Thromb. 1994 Jul;14(7):1056-65. doi: 10.1161/01.atv.14.7.1056.
8
Reverse cholesterol transport in plasma of patients with different forms of familial HDL deficiency.不同类型家族性高密度脂蛋白缺乏症患者血浆中的胆固醇逆向转运
Arterioscler Thromb Vasc Biol. 1995 May;15(5):691-703. doi: 10.1161/01.atv.15.5.691.
9
Two-dimensional electrophoresis of plasma lipoproteins: recognition of new apo A-I-containing subpopulations.血浆脂蛋白的二维电泳：新型含载脂蛋白A-I亚群的识别
Biochim Biophys Acta. 1993 Sep 8;1169(3):291-300. doi: 10.1016/0005-2760(93)90253-6.
10
High density lipoprotein metabolism.高密度脂蛋白代谢
J Lipid Res. 1984 Oct;25(10):1017-58.