Atrah H I, Ahmed M M
West Midlands Blood Transfusion Centre, Edgbaston, Birmingham.
J Clin Pathol. 1996 Mar;49(3):254-5. doi: 10.1136/jcp.49.3.254.
The significance of seroconversion as detected by an ELISA screening test for hepatitis C virus (HCV) antibody with a negative supplemental/confirmatory recombinant immunoblot assay (RIBA) result was investigated. Of 118,220 established West Midlands blood donors with at least one negative HCV antibody screen, 43 had seroconverted in 1994 according to the ELISA but had negative RIBA-3 results. The paired archive serum samples of the pre- and postseroconversion donations of 29 seroconverting donors were tested by nested polymerase chain reaction (PCR) for the detection of HCV RNA. All 58 samples were negative by PCR. The absence of detectable viraemia in all tested seroconverting donors suggests that HCV infection was not responsible for seroconversion by ELISA.
研究了通过丙型肝炎病毒（HCV）抗体酶联免疫吸附试验（ELISA）筛查检测到的血清转化，以及补充/确证重组免疫印迹试验（RIBA）结果为阴性的意义。在西米德兰兹郡已登记的118220名至少有一次HCV抗体筛查结果为阴性的献血者中，根据ELISA检测，有43人在1994年出现了血清转化，但RIBA - 3结果为阴性。对29名发生血清转化的献血者血清转化前后配对的存档血清样本进行巢式聚合酶链反应（PCR）检测，以检测HCV RNA。所有58个样本的PCR检测结果均为阴性。所有检测的发生血清转化的献血者均未检测到病毒血症，这表明ELISA检测到的血清转化并非由HCV感染引起。
