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通过差速离心法从组织和细胞中分离线粒体。

Isolation of mitochondria from tissues and cells by differential centrifugation.

作者信息

Graham J M

机构信息

Liverpool John Moores University, Liverpool, United Kingdom.

出版信息

Curr Protoc Cell Biol. 2001 May;Chapter 3:Unit 3.3. doi: 10.1002/0471143030.cb0303s04.

DOI:10.1002/0471143030.cb0303s04
PMID:18228355
Abstract

Differential centrifugation is used to prepare a "heavy" mitochondrial fraction from liver. These mitochondria are relatively pure, highly coupled, and suitable for respiratory studies. This unit presents protocols for isolation of beef heart mitochondria (also suitable for respiratory studies), skeletal muscle mitochondria, and mitochondria from cultured cells can also be isolated from homogenates by differential centrifugation. Differential centrifugation, which separates cellular organelles based on sedimentation velocity, is a rapid method for preparing mitochondria for metabolic studies.

摘要

差速离心法用于从肝脏中制备“重”线粒体组分。这些线粒体相对纯净、偶联性高，适用于呼吸研究。本单元介绍了分离牛心线粒体（也适用于呼吸研究）、骨骼肌线粒体的方案，培养细胞的线粒体也可通过差速离心法从匀浆中分离得到。差速离心法基于沉降速度分离细胞器，是一种为代谢研究制备线粒体的快速方法。

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