Zervos E E, Bass S S, Robson M C, Rosemurgy A S
Department of Surgery, University of South Florida, Tampa, USA.
J Trauma. 1996 Jul;41(1):10-4. doi: 10.1097/00005373-199607000-00003.
Candida infections after injury are associated with significant mortality. Death often results from gram negative sepsis. Because antifungal therapy lowers mortality seen with Candida infections, this study was undertaken to determine whether fluconazole (FCZ) augments host response against bacteria, specifically, the bactericidal activity of polymorphonuclear neutrophils (PMN).
PMN from 23 volunteers were incubated with phosphate-buffered saline or FCZ (20 micrograms/mL or 40 micrograms/mL). Bactericidal activity of these PMN, as well as FCZ alone, was determined using Escherichia coli and a colony forming unit protocol.
PMN possess potent bactericidal activity while FCZ possesses minimal inherent bactericidal activity. PMN bactericidal activity is significantly augmented by preincubation with FCZ. This enhanced bactericidal state is greater than the combined individual bactericidal properties of either FCZ concentration and PMN.
FCZ augments PMN bactericidal activity. This augmentation may help to explain the improved survival of critically injured patients receiving FCZ.
受伤后念珠菌感染与显著的死亡率相关。死亡通常由革兰氏阴性菌败血症导致。由于抗真菌治疗可降低念珠菌感染的死亡率，因此开展本研究以确定氟康唑（FCZ）是否能增强宿主对细菌的反应，特别是多形核中性粒细胞（PMN）的杀菌活性。
将来自23名志愿者的PMN与磷酸盐缓冲盐水或FCZ（20微克/毫升或40微克/毫升）一起孵育。使用大肠杆菌和菌落形成单位方案测定这些PMN以及单独的FCZ的杀菌活性。
PMN具有强大的杀菌活性，而FCZ具有最小的固有杀菌活性。通过与FCZ预孵育，PMN的杀菌活性显著增强。这种增强的杀菌状态大于FCZ任一浓度和PMN单独杀菌特性的总和。
FCZ增强PMN的杀菌活性。这种增强作用可能有助于解释接受FCZ治疗的重症受伤患者生存率的提高。
