粒细胞巨噬细胞集落刺激因子对中性粒细胞、单核细胞或单核细胞衍生巨噬细胞杀念珠菌活性的影响以及与氟康唑的协同作用。
Effect of granulocyte-macrophage colony-stimulating factor on candidacidal activity of neutrophils, monocytes or monocyte-derived macrophages and synergy with fluconazole.
作者信息
Natarajan U, Randhawa N, Brummer E, Stevens D A
机构信息
Department of Medicine, Santa Clara Valley Medical Center and California Institute for Medical Research, San Jose 95128-2699, USA.
出版信息
J Med Microbiol. 1998 Apr;47(4):359-63. doi: 10.1099/00222615-47-4-359.
The effect of in-vitro granulocyte-macrophage colony-stimulating factor (GM-CSF) treatment of neutrophils, monocytes or monocyte-derived macrophages (MDM) on candidacidal activity was tested. Synergy of effector cells with fluconazole (FCZ) for enhanced killing was also investigated. Incubation of neutrophils with GM-CSF 0.67 microg/L plus Candida albicans Sh27 for 24 h significantly increased candidacidal activity (36% versus 74%). Synergy with FCZ for killing was also significantly increased from 93% to 97% when neutrophils were incubated with GM-CSF. Monocytes cultured with GM-CSF and C. albicans for 24 h had significantly increased fungistatic activity compared to controls and synergy with FCZ for killing was significantly enhanced from 40% to 59%. Monocytes cultured for 3 days with GM-CSF had increased fungistatic activity compared to control MDM and showed synergy with FCZ for significantly enhanced killing (46%) compared to control MDM (12%).
测试了体外粒细胞-巨噬细胞集落刺激因子（GM-CSF）对中性粒细胞、单核细胞或单核细胞衍生巨噬细胞（MDM）进行处理后对其杀念珠菌活性的影响。还研究了效应细胞与氟康唑（FCZ）协同增强杀伤作用的情况。将中性粒细胞与0.67μg/L的GM-CSF以及白色念珠菌Sh27一起孵育24小时，显著提高了杀念珠菌活性（从36%提高到74%）。当中性粒细胞与GM-CSF一起孵育时，与FCZ协同杀伤的作用也从93%显著提高到97%。与对照相比，用GM-CSF和白色念珠菌培养24小时的单核细胞的抑菌活性显著增加，并且与FCZ协同杀伤的作用从40%显著增强到59%。与对照MDM相比，用GM-CSF培养3天的单核细胞的抑菌活性增加，并且与FCZ协同杀伤的作用显著增强（46%），而对照MDM的协同杀伤作用为12%。
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