Brummer E, Stevens D A
Department of Medicine, Santa Clara Valley Medical Center, CA, USA.
Mycopathologia. 1996;134(3):115-20. doi: 10.1007/BF00436717.
The killing of Candida species by human neutrophils in a long-term 24-h assay and possible synergy with fluconazole (FCZ) for killing was investigated. The test medium (TM) consisted of RPMI-1640, penicillin and streptomycin (P/S), and 10% fresh autologous serum. TM alone was highly fungistatic for Candida species compared to TM without serum. When neutrophils were cocultured in TM with Candida species for 24 h the inoculum colony-forming units (CFU) were always significantly reduced (killing) by 58 to 99%. FCZ was tested over a range of 1-500 micrograms/ml, and though almost always fungistatic itself, it synergized with neutrophils for significantly increased killing of C. albicans (isolate Sh27) (P < 0.01) and C. albicans (isolate 94-20) (P < 0.05). Killing of non-albicans Candida species was so efficient in the absence of FCZ that demonstration of synergy with FCZ was difficult.
在一项为期24小时的长期试验中，研究了人类中性粒细胞对念珠菌属的杀伤作用以及与氟康唑（FCZ）协同杀伤的可能性。试验培养基（TM）由RPMI-1640、青霉素和链霉素（P/S）以及10%新鲜自体血清组成。与无血清的TM相比，单独的TM对念珠菌属具有高度抑菌作用。当中性粒细胞与念珠菌属在TM中共培养24小时时，接种菌的菌落形成单位（CFU）总是显著减少（杀伤）58%至99%。在1-500微克/毫升的范围内对FCZ进行了测试，尽管它本身几乎总是抑菌的，但它与中性粒细胞协同作用，显著增强了对白色念珠菌（分离株Sh27）（P<0.01）和白色念珠菌（分离株94-20）（P<0.05）的杀伤作用。在没有FCZ的情况下，对非白色念珠菌的杀伤效率很高，因此很难证明与FCZ的协同作用。
