Sadowski J, Gaubier P, Delseny M, Quiros C F
Department of Vegetable Crops, University of California, Davis 95616, USA.
Mol Gen Genet. 1996 Jun 12;251(3):298-306. doi: 10.1007/BF02172520.
We report the genetic and physical analysis by pulse field gel electrophoresis (PFGE) in three Brassica diploid genomes for a cluster of five genes characterized in a selected segment of 15 kb on chromosome 3 of Arabidopsis thaliana, encoding a Bradyrhizobium CycJ homologue (At1), a rat p67 translation factor homologue (At2), an Em-like (early methionine) protein (At3), chlorophyll synthase (At4) and a yeast Sac1 homologue (A5). The Arabidopsis gene array was found to be conserved on a single linkage group in each of the Brassica genomes. However, partial complexes were found to be duplicated in other chromosome segments on the same or other linkage groups. Some of the At genes, which could not be genetically mapped because of lack of polymorphism, were assigned to their respective linkage groups by physical mapping. The presence of multiple copies of the A. thaliana gene cluster in the three Brassica genomes further establishes their complex nature, which results from extensive duplication and chromosomal rearrangement. In general, genetic distances between the At genes agreed with values expected for the physical distances determined in Brassica.
我们通过脉冲场凝胶电泳（PFGE）对三个芸苔属二倍体基因组进行了遗传和物理分析，研究对象是拟南芥3号染色体上一段15 kb选定片段中的五个基因簇，这些基因分别编码慢生根瘤菌CycJ同源物（At1）、大鼠p67翻译因子同源物（At2）、Em样（早期甲硫氨酸）蛋白（At3）、叶绿素合酶（At4）和酵母Sac1同源物（A5）。发现拟南芥基因阵列在每个芸苔属基因组的单个连锁群上是保守的。然而，部分复合体在同一或其他连锁群的其他染色体片段中存在重复。一些由于缺乏多态性而无法进行遗传定位的拟南芥基因，通过物理定位被分配到各自的连锁群中。三个芸苔属基因组中存在多个拟南芥基因簇拷贝，进一步证实了它们的复杂性质，这是由广泛的重复和染色体重排导致的。总体而言，拟南芥基因之间的遗传距离与芸苔属中确定的物理距离预期值相符。
