Chung Y D, Kwon H C, Chung K W, Kim S J, Kim K, Lee C C
Department of Molecular Biology, Seoul National University, Korea.
Mol Gen Genet. 1996 Jun 12;251(3):347-51. doi: 10.1007/BF02172525.
It has been reported that three different DNA regions-the fat body enhancer and ovarian enhancers 1 and 2-direct the tissue-specific expression of yp1 and yp2 in Drosophila melanogaster. In the present study, we identified ovarian enhancer 1-specific binding proteins. Electrophoretic mobility shift assay revealed that these proteins are present in the adult ovary, but not in adult testis or fat body. Southwestern blot analysis showed that about 130 kDa and 40 kDa proteins, designated OEF1 and OEF2, respectively, from ovarian nuclear or crude extracts bind specifically to the ovarian enhancer 1. The two proteins were partially purified by streptavidin/agarose-DNA affinity chromatography, and their binding activity was confirmed by electrophoretic mobility shift assay. These ovarian enhancer factors may play an important role in the regulation of transcription of yp1 and yp2 in the ovary.
据报道，在黑腹果蝇中，三个不同的DNA区域——脂肪体增强子以及卵巢增强子1和2——指导着yp1和yp2的组织特异性表达。在本研究中，我们鉴定出了卵巢增强子1特异性结合蛋白。电泳迁移率变动分析表明，这些蛋白存在于成年卵巢中，但不存在于成年睾丸或脂肪体中。蛋白质印迹分析显示，来自卵巢核提取物或粗提物的分别命名为OEF1和OEF2的约130 kDa和40 kDa的蛋白特异性结合卵巢增强子1。这两种蛋白通过链霉亲和素/琼脂糖-DNA亲和层析进行了部分纯化，其结合活性通过电泳迁移率变动分析得以证实。这些卵巢增强子因子可能在卵巢中yp1和yp2转录的调控中发挥重要作用。
