Muramatsu H, Chiba N, Nakajima K, Ataka T, Fujihira M, Hitomi J, Ushiki T
Research Lab. for Advanced Tech., Seiko Instruments Inc., Chiba, Japan.
Scanning Microsc. 1996;10(4):975-82.
We have developed scanning near-field optical/atomic force microscopy (SNOM/AFM). The SNOM/AFM uses a bent optical fiber simultaneously as a dynamic force AFM cantilever and a SNOM probe. Resonant frequency of the optical fiber cantilever is 15-40 kHz. Optical resolution of the SNOM/AFM images shows less than 50 nm. The SNOM/AFM system contains photon counting system and polychrometer/intensified coupled charge devise (ICCD) system to observe fluorescence image and spectrograph of micro areas, respectively. Cultured cells were stained with fluorescein isothiocyanate (FITC)-labeled anti-keratin antibody or FITC-labeled phalloidin after treatment with Triton X-100. Fluorescence and topographic images were obtained in air and water. The fluorescence images showed clear images of keratin and actin filaments. The SNOM/AFM is perfect to observe biomaterials in liquid with a liquid chamber while the topographic Images showed subcellular structures which correspond to keratin and actin filaments.
我们已经开发出扫描近场光学/原子力显微镜（SNOM/AFM）。该SNOM/AFM使用一根弯曲的光纤同时作为动态力原子力显微镜的悬臂和SNOM探针。光纤悬臂的共振频率为15 - 40千赫兹。SNOM/AFM图像的光学分辨率小于50纳米。SNOM/AFM系统包含光子计数系统和多色仪/增强型电荷耦合器件（ICCD）系统，分别用于观察微区的荧光图像和光谱。用Triton X - 100处理后，培养细胞用异硫氰酸荧光素（FITC）标记的抗角蛋白抗体或FITC标记的鬼笔环肽染色。在空气和水中获得荧光图像和形貌图像。荧光图像清晰显示了角蛋白和肌动蛋白丝的图像。SNOM/AFM非常适合在带有液体腔的液体中观察生物材料，而形貌图像显示了与角蛋白和肌动蛋白丝相对应的亚细胞结构。
