东方田鼠中一个含B1样重复序列的组织与染色体定位
Organization and chromosomal localization of a B1-like containing repeat of Microtus subarvalis.
作者信息
Mayorov V I, Adkison L R, Vorobyeva N V, Khrapov E A, Kholodhov N G, Rogozin I B, Nesterova T B, Protopopov A I, Sablina O V, Graphodatsky A S, Zakian S M
机构信息
Institute of Cytology and Genetics, Siberian Department of the Russian Academy of Sciences, Novosibirsk Russia 630090, USA.
出版信息
Mamm Genome. 1996 Aug;7(8):593-7. doi: 10.1007/s003359900176.
A repetitive DNA sequence, MS2, was isolated from EcoRI-digested genomic DNA of the vole, Microtus subarvalis. The fragment was cloned and sequenced. Sequence analysis of this 1194-bp fragment revealed a 156-bp region demonstrating a 55% homology with the mouse B1 repeat. The remaining MS2 sequence shows no significant homology with other known GenBank sequences. The results of in situ hybridization of MS2 on vole metaphase chromosomes indicate the fragment is confined to heterochromatin blocks of the sex chromosomes in all but one species (M. arvalis). Distribution of MS2 sequences provides evidence for heterogeneity of the giant heterochromatin blocks of the XY Chromosomes (Chrs) in voles, for the unique cluster-like localization of MS2 within these blocks.
从田鼠（Microtus subarvalis）经EcoRI酶切的基因组DNA中分离出一个重复DNA序列MS2。该片段被克隆并测序。对这个1194碱基对片段的序列分析显示，有一个156碱基对的区域与小鼠B1重复序列有55%的同源性。其余的MS2序列与GenBank中其他已知序列没有显著同源性。MS2在田鼠中期染色体上的原位杂交结果表明，除了一个物种（M. arvalis）外，该片段局限于性染色体的异染色质区。MS2序列的分布为田鼠XY染色体（Chrs）巨大异染色质区的异质性提供了证据，也为MS2在这些区域内独特的簇状定位提供了证据。
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