Easty D J, Maung K, Lascu I, Véron M, Fallowfield M E, Hart I R, Bennett D C
Department of Anatomy and Developmental Biology, St George's Hospital Medical School, London, UK.
Br J Cancer. 1996 Jul;74(1):109-14. doi: 10.1038/bjc.1996.323.
NM23 is a putative metastasis-suppressor gene for some human cancers. Here we have studied NM23 expression during melanoma progression using Northern blotting and immunocytochemistry. There was no significant difference in the average amounts of NM23 mRNA between cell lines derived from metastatic and primary melanomas. The level of NM23 mRNA was also determined for three pairs of poorly metastatic parental (P) and their highly metastatic variant (M) cell lines; the ratios for M/P were 1.2, 0.98 and 0.80. Next we used immunocytochemistry to study NM23 protein in normal skin, benign naevi and primary and metastatic melanomas. Melanocytes in all normal skin and benign samples were positive for NM23; however most primary melanomas (7/11) were not stained by the antibody. All metastatic melanoma samples (5/5) were positively stained. Findings were similar with an antiserum reactive with both forms of NM23 (H1 and H2), and with an antibody specific for NM23-H1. No relationship was apparent between NM23 immunoreactivity in primary tumours and their aggressiveness or prognosis. Hence, in contrast to the situation described for murine melanoma, the amount of NM23 mRNA or protein in human melanoma did not correlate inversely with metastasis.
NM23是一种推测的某些人类癌症转移抑制基因。在此，我们利用Northern印迹法和免疫细胞化学研究了黑色素瘤进展过程中NM23的表达情况。源自转移性和原发性黑色素瘤的细胞系之间，NM23 mRNA的平均含量没有显著差异。我们还测定了三对低转移性亲本（P）及其高转移性变体（M）细胞系的NM23 mRNA水平；M/P的比值分别为1.2、0.98和0.80。接下来，我们利用免疫细胞化学研究了正常皮肤、良性痣以及原发性和转移性黑色素瘤中的NM23蛋白。所有正常皮肤和良性样本中的黑素细胞NM23均呈阳性；然而，大多数原发性黑色素瘤（7/11）未被该抗体染色。所有转移性黑色素瘤样本（5/5）均呈阳性染色。使用与两种形式的NM23（H1和H2）均反应的抗血清以及对NM23-H1特异的抗体，得到了相似的结果。原发性肿瘤中NM23的免疫反应性与其侵袭性或预后之间没有明显关系。因此,与小鼠黑色素瘤的情况相反，人类黑色素瘤中NM23 mRNA或蛋白的量与转移没有负相关。
