Study of cellulose acetate membrane-based glucose biosensors.

A new immobilization procedure was designed to covalently immobilize enzymes onto a cellulose acetate membrane that was simple and easy to handle. The resultant enzyme membrane was very stable, with high specific activity. A glucose oxidase membrane was prepared by using this method, which was combined either with an oxygen electrode or with a hydrogen peroxide electrode to construct a glucose biosensor. The response characteristics of both biosensors were compared.