Wang S, Ji X, Yuan Z
Shanghai Institute of Biochemistry, Chinese Academy of Sciences, China.
Chin J Biotechnol. 1995;11(3):199-205.
A new immobilization procedure was designed to covalently immobilize enzymes onto a cellulose acetate membrane that was simple and easy to handle. The resultant enzyme membrane was very stable, with high specific activity. A glucose oxidase membrane was prepared by using this method, which was combined either with an oxygen electrode or with a hydrogen peroxide electrode to construct a glucose biosensor. The response characteristics of both biosensors were compared.
设计了一种新的固定化程序，用于将酶共价固定在易于操作的醋酸纤维素膜上。所得的酶膜非常稳定，具有高比活性。使用该方法制备了葡萄糖氧化酶膜，该膜与氧电极或过氧化氢电极结合以构建葡萄糖生物传感器。比较了两种生物传感器的响应特性。
